Structure , Attributive and Bacterial Effectiveness Studies for Complexes , Co ( II ) , Hg ( II ) , Ni ( II ) , Cu ( II ) and Mn ( II ) with Ligand kind ( N 2 O 2 )

Rehab K. Alshemary Faeza H. Ghanim Dept. of Chemistry/ College of Education for Pure Science (Ibn -Al-Haitham)/ University of Baghdad Received in:1/March/2016,Accepted in:28/June/2016 Abstract Binuclear copper, nickel, cobalt, manganese and mercury complexes of the Schiff base H2L (C40H28N2O4) obtained by condensation of 2-benzoyl benzoic acid with benzidine. The Schiff base and their complexes have been subjected to[ FT-IR, elemental analysis ,UV-Vis, H and C NMR ] spectral studies, molar conductivity, magnetic moment and HPLC measurements. All the complexes showed tetrahedral geometries with the general structure [M2(L)2]. Generality of the synthesized components offer antibacterial efficiency to (Staphylococcu saureus), (Escherichia coli),(Bacillus subtilis) and(Pseudomonas aeruginosa).


Introduction
The Ligand such as Schiff bases have a substantial part in a many of uses in biochemical and physicochemical [1] biochemical pertinent researches of metal complexes and given broad range of applications such as antipyretic [2],analgesic [3],anti-diabetic [4], antiinflammatory [5],anti-cancer [6] anti-human immunodeficiency virus activity(HIV)] [7] and anti-bacterial [8].Coordinating ligands including O and N giver atoms appear wide biological activity of special attention because of the set of ways in which they are bonded to metal ions [9].It is well known that different Schiff base complexes have Tetra-chelating N,N,O,O-giver Schiff bases in their nickel(II) complex avail as substantial models for enzyme [10].Their binuclear homometallic copper (II),nickel(II) and cobalt(II) complexes avail as model compounds at the active groups of metalloenzymes [12].Binuclear metal complexes find applications as active appliances for estimation and structure of outer kinds [13].Thus, functional and designing structural binuclear complexes keep a topic of continuous investigation.Tetradentate Schiff bases derived from benzoic acid with benzidine and its derivatives have been extensively explored; while Schiff bases obtained from 2-benzyl benzoic acid with benzidine and its derivatives has been searched very littler [14].In this paper, we report the preparation, structural, spectral, and description of a new binuclear the copper (II), nickel(II), cobalt(II), mercury (II) and manganese(II)complex [M(L)] 2 ,derived from the [H 2 L] obtained by condensation of [2-benzoylbenzoic acid and benzidine] (Scheme1).Structure, attributive and bacterial effectiveness studies detect that five metal (II) ions select tetrahedral configurations.

Experimental
Materials: All chemicals and all metal (II) chlorides 2-benzoyl benzoic acid, solvents and benzidine used in this work were obtained from Merck products.

Preparation The Ligand
The compound was synthesized from the condensation (1mmol) benzidine with (2mmol) 2benzoylbenzoic acid and (3) drops of glacial acetic acid in 25ml ethanol absolute (scheme 1).This reaction was refluxed at (75 • C) during 5hrs on water bath.A yield isolated out on refrigeration, was filtered off.A product result was got then re-crystallized from a hot solution of [(1ml) distilled water, (3ml) methanol and (2ml) acetone].This result was then submitted to chromatography "flash" as dry column by ethanol as eluent, yield (0.24g, 79%), m.p. (170)°.

Study of Biological Efficiency
The antibacterial activity for these compounds was evaluated by using method similar with the traditional disc diffusion method [7 and 8], sterile 5 mm filter paper discs (What man, no.1) were soaked in this compound (Disc loaded with the DMSO and allowed until complete evaporation to be used.Then discs were placed onto the surface of the Muller Hinton agar plates at different areas on the surface of each plate, after a 24 hrs.culture of the pathogenic strains(Staphylococcus usurers),(Escherichia coli),(Bacillus btilis) and (Pseudomonas aeruginosa) were spreading with a antiseptic cotton wipe over the surface of Muller Hinton agar plates.The plates were incubated for 24 hrs at 37C o .Products were fixed by registering the diameter in (mm) for a region of restraint on each plate.
The conductance [non-electrolytic nature] specified of the complexes specified [11].The magnetic sensitivity for these complexes (Table 5) in ethanolic which has been adduced for utmost tetrahedral arrangement [12].
The UV-Vis spectra in (10 -3 M) ethanolic solution of the ligand offered fundamentally two absorptions at 289 and 343 nm were spotted to the middle energy π→ π * and n→ π * transition Figure (5) [13 and 14].The UV-Vis spectrum of Mn(II) complex displayed absorptions at (297 and 365 nm) were regarded to L.F and C.T transitions respectively, and other absorptions at 487 and 584 nm were regarded to{ 6 A 1 → 4 A 1(G) and 6 A 1 → 4 E (G) }, separately [15] .The UV-Vis spectrum of Co(II) complex offered absorptions at 292 and 347 nm due to L.F and C.T transitions, other peak at 621 nm regarded to electronic transition type { 4 A 2(F) → 4 T 1(P) } .The spectrum of Ni (II) complex displayed absorptions at 284 and 346 nm result to L.F and C.T transition respectively.The peaks at 419 nm and 702 nm were result to (d-d) electronic transition types [16] { 3 T 1 → 3 T 1 and 3 T 1 → 3 A 2 } Figure (6) .The spectrum of Cu(II) complex showed peaks at 290 and 340 nm result to L.F and C.T transitions respectively, then other peak at 509 nm was described to electronic transition type[17] 2 T 2 → 2 E.The spectrum of complex displayed absorptions at 310 and 388 nm result to C.T transitions.The non-attendance of peaks in the Vis-area specified no (d-d) transitions showed tetrahedral geometry Table 5 [18].
The IR-spectrum of the ligand displayed frequency at 3441 cm -1 , which was described to υ (OH) group [19].This frequency was lost in the spectra of these complexes [20].A considerable shift in the in position intensity to lower wave number was noticed in all the complexes with metal ion for the strong frequency in the (H 2 L)spectrum, which shows at 1681 cm -1 described to υ(C=N) group [21].An important change in position to lower wave number and in the intensity was noticed with metal ion on complexation for the strong frequency in the ligand (H 2 L) spectrum at 1535 cm -1 , which appears due to υ as (COO -) [22].The band at 1367 cm -1 in the spectrum of the ligand (H 2 L), which was described to the υ s (COO -).These frequencies moved to lower or higher vibrations at (1496 -1525cm -1 ) and (1367-1404cm -1 ) for all the complexes, Δυ=(92-132) cm -1 [23].The occurrence of new bands in the range (484-571)cm -1 are temporarily designated to (Metal-Ligand) υ(M-O) and υ(M-N) frequencies [24], giving to the data gained, a tetrahedral arrangement has been temporarily given these complexes.The antimicrobial efficiencies of the compounds have been examined versus chosen kind of microorganism; the region of suppression of bacterial evolution on the plate.DMSO (which was used as a solvent and Controller) was a good solvent to prepare 1ppm of each tested sample Figure (7) [25 and 26].Table (6).