Antioxidant, Antimicrobial and Spectroscopic Discussion of Guanine Azo Ligand with Cu(II) and Ag (I) Complexes

In this paper, we have provided a very thorough analysis of a new novel chelate metal ion complex of [Cu(II),Ag(I)] prepared via the interaction with the ligand{ 2-amino-8-((4-chloro-3-hydroxyphenyl) diazenyl)azo]guanine} [LAAG], which is synthesized by diazo coupling of the 5-amino-2-chlorophenol with amino acid guanine. The ligand and its complexes are identified by a variety of techniques, like [HNMR, FTIR, and Uv-vis] spectral, thermal analysis (TGA), and element analyses (CHN). The molar ratio was achieved so that the Cu(II) complex has (1:2) (M:L) with octahedral geometry; however, the Ag(I) complex has (1:1) (M:L) with tetrahedral geometry, and the ligand acts as neutral N,N-bidentate; as well as the ligand (LAAG) and its complexes were assessed against the two types of bacteria ( Klebsiella pneumonia, Staphylococcus aureus ,antifungal (Candida), and antioxidant This study showed that all compounds (the ligand and its complexes) had antimicrobial activity and more biological activity.


Introduction
The synthesis of azo dye derivatives incorporating heterocycles as possible scaffolds is currently receiving a lot of attention in the pharmaceutical industry.A straightforward synthetic method that can produce a variety of azo dye derivatives is required by pharmaceuticals and medical drugs.The target derivatives' bioactive characteristics have been enhanced by the addition of the heterocyclic moiety to the azo dye scaffold.By adding heterocyclic moieties, it is simple to adjust the many biological and pharmacological applications of medications, such as their anti-fungal and anti-bacterial characteristics.To this day, attempts are still being undertaken to find better, more effective, and safe synthesis processes for azo dye derivatives.They are the subject of several scientific studies due to their uses as indicators, therapeutic compounds, and textile dyes.Because they possess biological characteristics such as antibacterial, antifungal, anti-HIV, and anticancer, azo dyes are significant in medicinal chemistry [1].They are, on the other hand, frequently used as biomaterial structural controllers, optical recording devices, photovoltaic devices, molecular switches, photo electronics, and printing systems.They also play a significant role in food and analytical chemistry.Heterocyclic azo dyes and their metal complexes are among these chemicals that can be engaged in biological reactions such as nitrogen-fixing and RNA inhibition.The identification of the ligand and its complexes was done using several physicochemical and spectroscopic methods.The antioxidant, antibacterial, antifungal, A chemical compound known as a color additive is one that combines chemically with another substance to produce a color.In the production of pharmaceuticals, a variety of organics and dyes are used.In the pharmaceutical industry [2], colors are used for financial, psychological, and practical reasons.Drugs can be distinguished by their colors to help patients understand their strengths, lowering the chance of an overdose or underdose.

E imental exper
Always the greatest equipment, materials, and solvent agents were employed.Using a Eure EA 3000 Elemental Analyzer, (C.H.N.) determines the elemental analyses and contents of chosen metal ions in the ligand (LAAG) and its complexes.A SHIMADZU, 8400s spectrophotometer was used to register FT-IR spectra in order to determine a sample's pH.CsI was used in the (250-4000) cm-1 range.The UV-Vis spectra of all the compounds were examined using the (SHIMADZU 1800 -UV-Vis spectrophotometer).The 1 H-NMR spectra were measured using a BRUKER AV 400 Avance-III (400 MHz and 100 MHz).The amount of metal in the produced ligand and complexes was quantified using thermal gravimetric analysis (TGA) (SDT, Q600 V20.9 Build).With the aid of Gallenkamp's melting point equipment, the melting points of each chemical were identified.The molar conductance of metal ion complexes was tested in unionized, pure water (10-3 M) (10-3 M) [25,26].The concentration of chloride in the complexes under examination was determined using the Mohr method.A Sherwood Scientific Auto Magnetic Susceptibility Balance Model was used to test the studied complexes' magnetic susceptibilities at room temperature.

Mole ratio
The most typical technique for ascertaining a complex's composition in solution is the mole ratio approach, which was employed in this case.The process is shown in Figure 1 together with the results of the location operations, and the data is shown in Table 2.The findings showed that the synthetic [Ag(LAAG)(H2O)2]NO3.2H2O complex has a

Thermogravimetric Analysis (TGA)
The projected and actual phase mass losses are listed in Table 3.In our investigation, argon flow was used to measure weight loss [2] and temperatures between (25 and 800 o C).Learning more about stoichiometry, thermal stability, and whether any compounds can be utilized to compute the decomposed species from the thermal graph are the main goals of thermal analysis [7, 8, and 9].4].Suggesting that no chelating occurred via these bonds [10], however, small alterations in location or form were occasionally attributed to a decrease or increase in resonance as a result of chelating [11].A B C cm-1.The posture and intensity of these bands were minified in the complex spectra by chelating [10,11] 4-A number of new bands were not present in the free ligand spectra when we were seen.However, the most noticeable changes were in the range [622, 405] cm-1.These bands, which appeared in this region, may be related to ʋ(M-Nazo), ʋ(M-Nimd) ʋ(M-O)H2O and ʋ(M-Cl).This will support our result as regards the chelation sites of the ligands with metal ions, and from the above, we conclude that the two ligands (LAAG) act as neutral N,N-bidentate ligands, forming a penta chelating ring [12].The 1H-NMR Spectra Figure 4 shows the chemical shift [] in (ppm) of the ligand (LAAG) and the Table was listed data (5) [13,14].
Table 5. 1 HNMR data for the LAAG ligand

Electronic spectrum of the (LAAG) ligand and its complexes and the magnetic proprieties
The electronic spectrum of the ligand (LAAG) in water [10 -4 M] with the range (280-1100)nm was given in Figure 5.It had two bands, the first band at(293nm,34129cm-1) which was associated with the intramolecular transition of heterocyclic and aromatic moieties [15] [24].The second band, which was seen at (339 nm, 29498 cm-1 ) ( n→π*), was attributed to an intramolecular charge transfer that occurred via the carbonyl and azo moieties.Sharp absorption bands in the electronic spectra of the dia magnetic (d 10 ) Ag(I), [25][29] complex were identified as the charge transfer C.T [16].

Conclusion
Two new metal complexes with novel azo ligands derived from guanine by the conventional diazo-coupling reaction Their molecular formulae were asserted by different spectroscopic methods such as [FT-IR, UV-Vis, HNMR ] spectra, thermal analyses (TGA), and C.H.N analyses.The present studies show that the ligand (LAAG) acts as neutral N, N-bidentate, and the geometry of the Cu-complex is distorted octahedral, while the Ag-complex has tetrahedral geometry.The ligand and its complexes have anti-bacterial, anti-fungal, and anti-oxidant properties.Hence, they may be used in pharmaceuticals to develop potential drugs.

Figure 1 .
Figure 1.Plot of absorbance against mole ratio of (LAAG) ligand and its complexes

Table 2 .
LAAG-Metal ion solution absorbance vs mole ratio

Table 3 .
the thermal stability of the synthesized compounds

Table 4 .
FTIR assignment bands for LAAG and their complexes