Glomerulogenesis and Histomorphometeric in Mus musculus Embryo

Authors

Amar Alzubaidi Department of Biology, Collage of Education Pure Science Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq.
Nahla A. Al-Bakri Department of Biology, Collage of Education Pure Science Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq.
Ateeq M. J. Alarami Department of Biology, Collage of Education, University of Thamar Republic of Yemen.

DOI:

https://doi.org/10.30526/36.2.3000

Keywords:

Keywords: Kidney, Mus musculus, Embryo, Ureteric bud, Glomerulus, Histomorphometry.

Abstract

In mammals, the kidneys originate in an embryo from the mesoderm through three excretory organs, namely: Pronephros, Mesonephros, and Metanephros. After the formation of Metanephros is completed, the kidneys begin to form nephrogenesis through mesenchymal cells located at the tip of the ureteric bud, that contribute in the formation of glomerulus and Renal tubules. The stages of glomerulus formation in the embryo of albino mice at the age of 14 to 19 days of gestation were studied. It was obtained after the sacrifice of the expectant mother and the kidneys were excised from the embryos and fixed using Aqueous Bouin's solution, Microscopic slices with a thickness of 6 microns were then made in a paraffin method and were photographed by a camera for microscopic imaging. Histological measurements were performed on them using the program IMAGE J program and analyzed statistically using the SPSS program.

Results of the current study showed the presence of five stages of glomerulus formation, namely, the renal vesicle, which represents the first stage in the formation of the nephron and glomerulus. This is followed by the Comma shape stage, S-shaped stage, Capillary loop stage and finally the Mature glomerulus. This is surrounded by a capsule, known as Bowman’s capsule being part of the Malpighian or Renal corpuscle. The statistical analysis showed that there were significant differences between the average diameters of the glomerular development stages, and that the mature glomerulus was larger in diameter than the rest of the stages. The study aims to determine the stages of glomerulus formation and histologically measure its diameter in the embryo of a Mus musculus.

References

Farquhar,M.G.;Wissing,S.L.; Palade,G.E. Glomerular permeability Ferritin transfer across the normal Glomerular capillary wall.Jor. am. Soc. Nephrol. 1999,(10),2645-2662.

Mescher, A.L. Junqueira’s Basic Histology Text and Atlas,15th edt; McGraw-Hill Education: New York, USA, 2018; ISBN: 978-1-26-002617-7

Martini ; Bartholomew; Ober, W.C.; Ober,C. E; Welch, K. ; Petti, K. Essentials of anatomy and physiology, 8thed; Pearson Education: Hoboken,USA, 2020; ISBN 0135210755.

Sadler, T.W. Langman's medical embryology, 14th. Wolters Kluwer, Philadelphia, USA, 2019; ISBN 9781496383907.

Moore, K.L.; Persaud,T.V.N., Torchia, M.G. The developing human: clinically oriented embryology,10th ed; Elsevier, Inc.: Philadelphia, USA, 2016; ISBN: 978-0-323-31338-4.

Al-Ibrahimi, L. H. ; Al-Bakri, N.A. Teratogenic Effect of Keppra (Levetiracetam) Drug on the Kidney in Albino Rat (Rattus rattus). IHJPAS 2017, 28(2),8-21.

Robert, B.; Zhao X, Abrahamson DR. Coexpression of neuropilin-1, flk1, and VEGF(164) in developing and mature mouse kidney glomeruli. Am. J. Physiol. Renal. Physiol. 2000, 279, 275–282

Al- Yassiri, S.J. ; Al-Bakri, N. ; Al-Kawaz, U. ; Selman,M. O. Histological Study on Kidney affected by Carbamazepine Drug in Postnatal Rat. World J. Pharm. Res. 2016,5(11), 205-219

Suvarna , S. K.; Layton, L. ; Bancroft, J. D. Bancroft's theory and practice of histological techniques, 7th ed; Churchill Livingstone Elsevier Ltd.: Shanghai, China,2019; ISBN 978-0-7020-6887-4.

Bancroft, J. D.; Suvarna, K. ; Layton, C. Bancroft Theoty and practice of histological techniques, 7th ed.;The Churcill Livingstone: Edinburgh, Scottish, 2012 ; ISBN 978-0-7020-4226-3.

Georgas,K.; Rumballe, B.; Valerius, M.T.; Chiu, H.S.; Thiagarajan, R.D.; Lesieur, E.; Aronow, B.J.; Brunskill, E.W.; Combes, A.N.; Tang, D.; Taylor, D.; Grimmond, S.M.; Potter, S.S.; McMahon, A.P.; Little, M.H. Analysis of early nephron patterning reveals a role for distal RV proliferation in fusion to the ureteric tip via a cap mesenchyme-derived connecting segment. Dev. Biol. 2009,332,273–286.

Syed,S.A ; Joshi, R. A. ; Herekar, N. G. Histogenesis of Kidney in Human Fetuses. Int. J. Rec. Trends Sci. Technol. 2012,3(2),44-48.

Faa, G.; Gerosa, C.; Fanni, D.; Monga, G.; Zaffanello, M.; Van Eyken, P.; Fanos, V. Morphogenesis and molecular mechanisms involved in human kidney development. J. Cell.Physiol.2011, 227(3), 1257–1268.

Nagata, M. Glomerulogenesis and the role of endothelium. Curr. Opin. Nephrol. Hypertens,2018, 27(3), 159–164.

Tanigawa, S.; Taguchi, A.; Sharma, N.; Perantoni, A. O.; Nishinakamura, R. Selective In Vitro Propagation of Nephron Progenitors Derived from Embryos and Pluripotent Stem Cells. Cell reports 2016, 15(4), 801–813.

Haroldsson, B.; Nystrom, J.; Deen, W.M. Properties of the glomerular basement barrier and mechanisms of proteinuria. Physiol. Rev. 2008, 88,451-487.

Pokarna, D. J.; Kshitija, K. ; Saritha, S. Histogenesis of human fetal kidney from 14 weeks to 36 weeks: a study. Int. J. Res. Med. Sci. 2019, 7(11),4330-4334.