Impact of Carbamazepine Treatment in Testicular Histology in Albino Mice (Mus musculus)

Authors

Zainab Abd-Ali Sabr Department of Biology College of Science for Women, University of Baghdad, Baghdad, Iraq
Zainab Kareem Department of biology , College of Science for Women , University of Baghdad, Baghdad, Iraq

DOI:

https://doi.org/10.30526/36.4.3128

Keywords:

Carbamazepine , testis , germ cells , Leydig cells, edema, necrosis

Abstract

Carbamazepine (CBZ) is an antiepileptic medication (AED) intended to treat epilepsy, although it is also used to treat psychiatric problems and neuropathic pain. CBZ use has been linked to male reproduction problems such as hormonal changes, sexual problems, and sperm quality loss in addition to its effect on the hypothalamic-pituitary-gonadal (HPG) axis. The present study aimed to check the potential consequences of carbamazepine on the histology of the testis. In this experiment, 24 adult males of Swiss albino mice were divided into four groups. The control group was gavaged with distilled water, and the others were treated with carbamazepine orally administered with 0.1 ml of a daily dose of concentrations (2.5, 5 and 10) mg/kg bw for 60 consecutive days. Several histological alterations were caused by the therapy in the testis, which included congestion, necrosis, edema, hemorrhage, shrinkage tubule, germ cell shedding, and amyloid.

References

Davico, C.; Canavese, C.; Vittorini, R.; Gandione, M.; Vitiello, B. Anticonvulsants for Psychiatric Disorders in Children and Adolescents: A Systematic Review of Their Efficacy. Front. Psychiatry, 2018; 9, 270.

Osuntokun, O.S.; Babatunde, A.A.; Olayiwola, G.; Atere, T.G.; Oladokun, O.O.; Adedokun, K.I. Assessment of the Biomarkers of Hepatotoxicity Following Carbamazepine, Levetiracetam, and Carbamazepine-Levetiracetam Adjunctive Treatment in Male Wistar Rats. Toxicol. Reports, 2021; 8, 592–598.

Erdem, S.A.; Nabavi, S.F.; Orhan, I.E.; Daglia, M.; Izadi, M.; Nabavi, S.M. Blessings in Disguise: A Review of Phytochemical Composition and Antimicrobial Activity of Plants Belonging to the Genus Eryngium. DARU J. Pharm. Sci., 2015; 23, 1–22.

Osuntokun, O.S.; Olayiwola, G.; Oladele, A.; Ola, I.; AyokaAbiodun, O. Chronic Administration of Gabapentin and a Gabapentin-Carbamazepine Combination Reversibly Suppress Testicular Function in Male Wistar Rats (Rattus Norvegicus). Pathophysiology, 2017; 24, 63–69.

Osuntokun, O.S.; Akinsomisoye, S.O.; Olayiwola, G.; Adedokun, K.I.; Oladokun, O.O. Carbamazepine Adversely Altered the Pituitary–Testicular Axis with Resultant Reproductive Dysfunctions than Levetiracetam or Carbamazepine–Levetiracetam Adjuvant Treatment in Male Wistar Rat. Andrologia, 2020; 52, e13871.

Karaduman, A.B.; Kilic, V.; Atli-Eklioglu, O.; Baysal, M.; Aydogan-Kılıc, G.; Ucarcan, S.; Ilgin, S. Reproductive Toxic Effects and Possible Mechanisms of Zonisamide in Male Rats. Hum. Exp. Toxicol. 2019; 38, 1384–1396.

Elkhayat, H.A.; Hamed, H.M.; Shouman, M.G.; Elagouza, I.A.; Mekkawy, L.H. Influence of Conventional Antiepileptic Drugs on Glutathione-S-Transferase and Lipid Peroxidation in Children with Idiopathic Epilepsy. Bull. Natl. Res. Cent., 2018; 42, 1–5.

Akorede, G.J.; Ambali, S.F.; Olatunji, A.O.; Shittu, M.; Aremu, A.; Basiru, A.; Biobaku, K.T.; Ahmed, A.O.; Ameen, S.A. Protective Effect of Vitamin C on Chronic Carbamazepine-Induced Reproductive Toxicity in Male Wistar Rats. Toxicol. Reports, 2020, 7, 269–276.

Suvarna, S.K.; Layton, C.; Bancroft, J.D. Theory and Practice of Histological Techniques. Pbl. London, Churchill Livingstone, Elsiver, 2013; 173–187.

Suvarna, K.S.; Layton, C.; Bancroft, J.D. Bancroft’s Theory and Practice of Histological Techniques E-Book; Elsevier health sciences, 2018; ISBN 0702068861.

O’donnell, L.; Robertson, K.M.; Jones, M.E.; Simpson, E.R. Estrogen and Spermatogenesis. Endocr. Rev., 2001; 22, 289–318.

O’Donnell, L.; McLachlan, R.I.; Wreford, N.G.; Robertson, D.M. Testosterone Promotes the Conversion of Round Spermatids between Stages VII and VIII of the Rat Spermatogenic Cycle. Endocrinology, 1994; 135, 2608–2614.

Singh, D.; Arya, P.V.; Aggarwal, V.P.; Gupta, R.S. Evaluation of Antioxidant and Hepatoprotective Activities of Moringa Oleifera Lam. Leaves in Carbon Tetrachloride-Intoxicated Rats. Antioxidants, 2014; 3, 569–591.

Okumura, K.; Lee, I.P.; Dixon, R.L. Permeability of Selected Drugs and Chemicals across the Blood-Testis Barrier of the Rat. J. Pharmacol. Exp. Ther., 1975; 194, 89–95.

Chen, S.; Shen, M.; Chen, T.; Lai, S. Effects of Antiepileptic Drugs on Sperm Motility of Normal Controls and Epileptic Patients with Long‐term Therapy. Epilepsia, 1992; 33, 149–153.

Miraglia, S.M.; Hayashi, H.; Goldfeder, E.M. Histomorfometria Dos Testículos de Ratos Albinos Normais Em Várias Idades. Rev. Bras. Ciênc. Morfol, 1990; 8–15.

Miraglia, S.M.; Hayashi, H. Histomorphometry of Immature Rat Testis after Heating. J. Morphol. 1993; 217, 65–74.

Freitas, F.E.L.; Cordeiro-Mori, F.; Sasso-Cerri, E.; Lucas, S.R.R.; Miraglia, S.M. Alterations of Spermatogenesis in Etoposide-Treated Rats: A Stereological Study. Interciencia, 2002; 27, 227–235.

Soliman, G.A.; Abd, A. Effects of Antiepileptic Drugs Carbamazepine and Sodium Valproate on Fertility of Male Rats. Dtsch. Tierarztl. Wochenschr, 1999; 106, 110–113.

Ibrahim, T.A.; Mohammed, M.A. Teratogenic Effect of Carbamazepine Drug on the Histological Structure of Testes in the Albino Mouse (Mus Musculus). Indian J. Forensic Med. Toxicol., 2020; 14, 1829–1834.

Svalheim, S.; Luef, G.; Rauchenzauner, M.; Mørkrid, L.; Gjerstad, L.; Taubøll, E. Cardiovascular Risk Factors in Epilepsy Patients Taking Levetiracetam, Carbamazepine or Lamotrigine. Acta Neurol. Scand., 2010; 122, 30–33.

Vijay, P.; Yeshwanth, R.; Bairy, K.L. Effect of Phenytoin Sodium on the Biochemical Parameters of Reproductive Function in Male Albino Wistar Rats. J. Physiol. Biomed. Sci., 2009; 22, 14–18.

Alaa, H. Histopathological Effects of Carbamazepine on The Reproductive System of Male Rats. Al-Qadisiyah J. Pure Sci., 2021; 26, 39–43.

Aliyu, M.; Saleh, M.I.A.; Alhassan, A.W.; Zuberu, J.; Adamu, B.Y.; Iliya, B.T.; Emmanuel, N.S. Evaluation of the Effect of Co-Administration of Resveratrol and Vitamin E on Carbamazepine-Induced Oxidative Stress in Male Adult Wistar Rats. J. African Assoc. Physiol. Sci., 2017; 5, 93–100.

Luck, M.R.; Jeyaseelan, I.; Scholes, R.A. Ascorbic Acid and Fertility. Biol. Reprod., 1995; 52, 262–266.

Baker, M.A.; Hetherington, L.; Weinberg, A.; Velkov, T. Phosphopeptide Analysis of Rodent Epididymal Spermatozoa. JoVE (Journal Vis. Exp., 2014; e51546.

Rao, M. V; Chinoy, N.J. Effect of Estradiol Benzoate on Reproductive Organs and Fertility in the Male Rat. Eur. J. Obstet. Gynecol. Reprod. Biol., 1983; 15, 189–198.

Saleh, R.A.; HCLD, A.A. Oxidative Stress and Male Infertility: From Research Bench to Clinical Practice. J. Androl. 2002, 23, 737–752.

Agarwal, A.; Virk, G.; Ong, C.; Du Plessis, S.S. Effect of Oxidative Stress on Male Reproduction. World J. Mens. Health, 2014; 32, 1–17.

Al–Timimi, Z.K. Metronidazole induces significant pathological alterations in the male reproductive system of mice. Iraqi J. Agric. Sci,. 2021; 52, 1375–1381.

Isojärvi, J.I.T.; Löfgren, E.; Juntunen, K.S.T.; Pakarinen, A.J.; Päivänsalo, M.; Rautakorpi, I.; Tuomivaara, L. Effect of Epilepsy and Antiepileptic Drugs on Male Reproductive Health. Neurology, 2004; 62, 247–253.