Anticancer and Antioxidant activity of Gold Nanoparticles Biosynthesized using Acinetobacter baumannii
DOI:
https://doi.org/10.30526/36.4.3141Keywords:
gold nanoparticles, Acinetobacter baumannii, antioxidant activity, anticancer activitiesAbstract
In the current study, gold nanoparticles were made using Acinetobacter baumannii broth culture. UV-vis, FTIR, XRD, FESEM, AMF, and zeta potential measurements were also used to study the properties of the Ab-AuNPs. The average was 66 nm, ranging from 20 to 90 nm. The examination results proved that the Ab-AuNPs are semi-spherical and varied from 20 to 90 nm, with an average of 66 nm.
MTT assay on the breast cancer cell line MCF-7 confirmed the anticancer activity in vitro. Cancer cells showed an important cytotoxic activity of Ab-AuNPs. The breast. Cancer cell. Line.MCF-7 but ineffective against the normal.cell line.MCF-10. The IC50 values of Ab-AuNPs were at 11.45 μg ml-1. The results proved that Ab-AuNPs have DPPH scavenging activity, which increases with increasing concentration of Ab-AuNPs, where the concentrations (3.1, 6.25, 12.5, 25, and 50) mg/m gave DPPH scavenging activity with the following values 37.87%, 50.13%, 59.33%, 75.55%, and 85.13% respectively.
The present study concludes that gold nanoparticles synthesized using A. baumannii broth culture are easy to prepare, inexpensive, and non-toxic to normal cells. Meanwhile, they possess antioxidant and anticancer activity. So, it can be used as an alternative treatment.
References
Katira, P.; Zaman, M. H.; Bonnecaze, R.T. How changes in cell mechanical properties induce
cancerous behavior. Physical Review Letters, 2021; 108, 2, 028103.
Bai Aswathanarayan, J.; Rai Vittal, R.; Muddegowda, U. Anticancer activity of metal
nanoparticles and their peptide conjugates against human colon anorectal carcinoma cells.
Artificial Cells, Nanomedicine, and Biotechnology, 2018; 46, 7, 1444-1451.
Sieja, K.; Talerczyk, M. Selenium is an element in treating ovarian cancer in women receiving
chemotherapy. Gynecologic Oncology, 2004; 93, 2, 320-327.
Elmore, J.G.; Armstrong, K.; Lehman, C.D.; Fletcher, S.W. Screening for breast cancer. Jama,
; 293, 10, 1245-1256.
Lerman, C.; Lustbader, E.; Rimer, B.; Daly, M.; Miller, S.; Sands, C.; Balshem, A. Effects of
individualized breast cancer risk counseling: a randomized trial. JNCI: Journal of the National
Cancer Institute, 1995; 87, 4, 286-292.
Khan, A.K.; Rashid, R.; Murtaza, G.; Zahra, A.J.T.R. Gold nanoparticles: synthesis and
applications in drug delivery. Tropical journal of Pharmaceutical Research, 2014; 13, 7, 1169-
Jadoun, S.; Chauhan, N.; Zarrintaj, P. Synthesis of nanoparticles using microorganisms and
their applications: A review. Environmental Chemistry Letters, 2022; 1-45.
Buzea, C.; Pacheco, I.I.; Robbie, K. Nanomaterials and nanoparticles: sources and toxicity.
Biointerphases, 2007; 2, 4, MR17-MR71.
Heidary, M.; Nasiri, M.J.; Dabiri, H.; Takashi, S. Prevalence of drug-resistant Klebsiella
pneumoniae in Iran: a review article. Iranian Journal of Public Health, 2018; 47, 3, 317.
Sabir, S.; Zahoor, M.A.; Waseem, M.; Siddique, M.H.; Shafique, M.; Imran, M.; Muzammil,
S. Biosynthesis of ZnO nanoparticles using bacillus subtilis: characterization and nutritive
significance for promoting plant growth in Zea mays L. Dose-Response, 2020; 18, 3,
Ali, I.H.; Jabir, M.S.; Al-Shmgani, H.S.; Sulaiman, G.M.; Sadoon, A.H. Pathological and
immunological study on infection with escherichia coli in ale balb/c mice. Journal of Physics:
Conference Series, 2018; 1003, 1, 012009.
IHJPAS. 36 (4) 2023
Khashan, K.S.; Badr, B.A.; Sulaiman, G.M.; Jabir, M.S.; Hussain, S.A. Antibacterial activity
of Zinc Oxide nanostructured materials synthesis by laser ablation method. Journal of Physics:
Conference Series, 2021; 1795, 1, 012040),
Al Salman, H.N.K.; Ali, E.T.; Jabir, M.; Sulaiman, G.M.; Al Jadaan, S.A. 2 Benzhydrylsulfinyl
N hydroxycarbamide Na extracted from fig as a novel cytotoxic and apoptosis inducer in
SKOV 3 and AMJ 13 cell lines via P53 and caspase 8 pathway. European Food Research and
Technology, 2020.
Jabir, M.; Sahib, U.I.; Taqi, Z.; Taha, A.; Sulaiman, G.; Albukhaty, S.; Rizwana, H. Linaloolloaded
glutathione-modified gold nanoparticles conjugated with CALNN peptide as apoptosis
inducer and NF-κB translocation inhibitor in SKOV-3 cell line. International Journal of
Nanomedicine, 2020; 15, 9025.
Younus, A.; Al-Ahmer, S.; Jabir, M. Evaluation of some immunological markers in children
with bacterial meningitis caused by Streptococcus pneumonia. Research Journal of
Biotechnology, 2019; 14, 131-133.
Ismail, E.H.; Saqer, A.M.; Assirey, E.; Naqvi, A.; Okasha, R.M. Successful green synthesis of
gold nanoparticles using a Corchorus olitorius extract and their antiproliferative effect in
cancer cells. International Journal of Molecular Sciences, 2018; 19, 9, 2612.
Langford, J.I.; Wilson, A.J.C. Scherrer after sixty years: a survey and new results in
determining crystallite size. Journal of Applied Crystallography, 1978; 11, 2, 102-113.
Al-Kalifawi, E.J. Silver Nanoparticles Synthesis by Hamza's Khubdat (AS)(Kombucha) Tea
and used in Burn Wounds Treatment. J. Glob. Pharma Technol., 2018; 10, 489.
Hawar, S.N. Extracellular Enzyme of Endophytic Fungi Isolated from Ziziphus spina Leaves
as Medicinal Plant. International Journal of Biomaterials, 2022.
Swami, A.; Mittal, S.; Chopra, A.; Sharma, R.K.; Wangoo, N. Synthesis and pH-dependent
assembly of isotropic and anisotropic gold nanoparticles functionalized with hydroxyl-bearing
amino acids. Applied Nanoscience, 2018; 8, 3, 467-473.
Santhoshkumar, J.; Rajeshkumar, S., Kumar, S.V. Phyto-assisted synthesis, characterization,
and applications of gold nanoparticles–A review. Biochemistry and Biophysics Reports, 2017;
, 46-57.
Swami, A.; Mittal, S.; Chopra, A., Sharma, R.K.; Wangoo, N. Synthesis and pH-dependent
assembly of isotropic and anisotropic gold nanoparticles functionalized with hydroxyl-bearing
amino acids. Applied Nanoscience, 2018; 8, 3, 467-473.
Akintelu, S.A.; Olugbeko, S.C.; Folorunso, A.S. A review of synthesis, optimization,
characterization, and antibacterial application of gold nanoparticles synthesized from plants.
International Nano Letters, 2020; 10, 4, 237-248.
Singaravelu, G; Arockiamary, J.S.; Kumar, V.G.; Govindaraju, K. A novel extracellular
synthesis of monodisperse gold nanoparticles using marine alga, Sargassum wightii Greville.
Colloids and surfaces B: Biointerfaces, 2007; 57, 1, 97-101.
Song, J.Y.; Jang, H.K.; Kim, B.S. Biological synthesis of gold nanoparticles using Magnolia
kobus and Diospyros kaki leaf extracts. Process Biochemistry, 2009; 44, 10, 1133-1138.
Zhang, X.; Qu, Y.; Shen, W.; Wang, J.; Li, H., Zhang, Z.; Zhou, J. Biogenic synthesis of gold
nanoparticles by yeast Magnusiomyces ingens LH-F1 for catalytic reduction of nitrophenols.
Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2016; 497, 280-285.
Sharma, N.; Pinnaka, A.K.; Raje, M.; Fnu, A.; Bhattacharyya, M.S.; Choudhury, A.R. The
exploitation of marine bacteria for the production of gold nanoparticles. Microbial Cell
Factories, 2012; 11, 1, 1-6.
IHJPAS. 36 (4) 2023
Luzala, M.M.; Muanga, C.K.; Kyana, J.; Safari, J.B.; Zola, E.N.; Mbusa, G.V.; Memvanga,
P.B. A Critical Review of the Antimicrobial and Antibiofilm Activities of Green-Synthesized
Plant-Based Metallic Nanoparticles. Nanomaterials, 2022; 12, 11, 1841.
Vitosha, M.; Palanisamy, S.; Muthukrishnan, R.; Selvam, S.; Kannapiran, E.; You, S.; Prabhu,
N.M. Biogenic synthesis of gold nanoparticles from Halymenia dilatata for pharmaceutical
applications: Antioxidant, anticancer, and antibacterial activities. Process Biochemistry, 2019;
, 219-229.
Jafarizad, A.; Safaee, K.; Gharibian, S.; Omidi, Y.; Ekinci, D. Biosynthesis and in-vitro study
of gold nanoparticles using Mentha and Pelargonium extracts. Procedia Materials Science,
; 11, 224-230.
Owaid, M.N.; Al-Saeedi, S.S.S.; Abed, I.A. Biosynthesis of gold nanoparticles using yellow
oyster mushroom Pleurotus cornucopiae var. citrinopileatus. Environmental Nanotechnology,
Monitoring & Management, 2017; 8, 157-162.
Aljabali, A.A.; Akkam, Y.; Al Zoubi, M.S.; Al-Batayneh, K.M.; Al-Trad, B.; Abo Alrob, O.;
Evans, D.J. Synthesis of gold nanoparticles using leaf extract of Ziziphus zizyphus and their
antimicrobial activity. Nanomaterials, 2018; 8, 3, 174.
Naser, D.K.; Abbas, A.K.; Aadim, K.A. Zeta potential of Ag, Cu, ZnO, CdO, and Sn
nanoparticles prepared by pulsed laser ablation in a liquid environment. Iraqi Journal of
Science, 2020; 2570-2581.
Maeh, R.K.; Jaaffar, A.I.; Al-Azawi, K.F. Preparation of Juniperus extract and detection of its
antimicrobial and antioxidant activity. The Iraqi Journal of Agricultural Science, 2019; 50, 3,
-1161.
Oh Abdullah, A.M. Green synthesis of aunps from the leaf extract of Prosopis fractal for
antibacterial and anti-cancer applications. Digest Journal of Nanomaterials and Biostructures,
; 15, 3, 943-951.
Yas, R. M., Ghafoor, A.; Saeed, M.A. Anticancer effect of green synthesized gold
nanoparticles using orchid extract and their characterizations on breast cancer AMJ-13 Cell
line. Syst. Rev. Pharm, 2021; 12, 500-505.
Hosny, M.; Fawzy, M.; Abdelfatah, A.M.; Fawzy, E.E.; Eltaweil, A.S. Comparative study on
the potentialities of two halophytic species in the green synthesis of gold nanoparticles and
their anticancer, antioxidant, and catalytic efficiencies. Advanced Powder Technology, 2021;
, 9, 3220-3233.
El Domany, E.B.; Essam, T.M.; Ahmed, A.E.; Farghali, A.A. Biosynthesis Physico-chemical
optimization of gold nanoparticles as anti-cancer and synergetic antimicrobial activity using
Pleurotus ostreatus fungus. Journal of Applied Pharmaceutical Science, 2018; 8, 5, 119-128.
Razzaq, H.; Saira, F.; Yaqub, A.; Qureshi, R.; Mumtaz, M.; Saleemi, S. Interaction of gold
nanoparticles with free radicals and their role in enhancing the scavenging activity of ascorbic
acid. Journal of Photochemistry and Photobiology B: Biology, 2016; 161, 266-272.
Desai, M.P.; Sangaokar, G.M.; Pawar, K.D. Kokum fruit-mediated biogenic gold nanoparticles
with photoluminescent, photocatalytic, and antioxidant activities. Process Biochemistry, 2018;
, 188-197.
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