Synthesis and Characterization of Proteolytic Enzyme Loaded on Silver Nanoparticles

Hanady Salim Al-Shmgani; Mohammed A.         Ashij; Khalil A. A.  Khalil; Hamdoon A.  Mohammed

doi:10.30526/37.1.3300

Authors

Hanady Salim Al-Shmgani Department of Biology, College of Education Pure Science Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq.
Mohammed A. Ashij Department of Biology, College of Education Pure Science Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq.
Khalil A. A. Khalil Department of Medical Laboratories Sciences, College of Applied Medical Sciences, University of Bisha, Bisha, Saudi Arabia.
Hamdoon A. Mohammed Department of Pharmacognosy, Faculty of Pharmacy, Al Azhar University, Cairo, Egypt.

DOI:

https://doi.org/10.30526/37.1.3300

Keywords:

Bromelain, antioxidant, silver nanoparticles, Turkevich method.

Abstract

Bromelain is a proteolytic enzyme rich in cysteine proteases, extracted from the stem and fruit of pineapple (Ananas comosus). There are several therapeutic applications of the bromelain enzyme, where it has anti-inflammatory, anti-cancer, and antimicrobial activity, reduces joint pain, and accelerates wound healing. In the current study, bromelain enzyme was loaded on silver nanoparticles (Br-AgNPs) prepared using the citrate-reduction Turkevich method. Different characterization analyses were performed, including UV-Vis spectrophotometers, FTIR, SEM, and XRD analyses. Moreover, the antioxidant activity of prepared Br-AgNPs was evaluated by DPPH assay. The results of UV-Vis showed a peak at 434 nm, which referred to the AgNPs formation, and FTIR results revealed groups of (C=O, C=C) at 1519.91 and 1539.20, respectively, and the amine group at 1384.89 and the flavonoids group at 1357.89. SEM results exhibit that the synthesized Br-AgNPs were spherical in shape, with average sizes of about 84.73 nm. Also, the AgNPs were crystalline in nature with face-centered cubic symmetry. The synthesized BR-AgNPs showed strong in vitro antioxidant activity in a dose-dependent manner. In conclusion, successfully synthesized silver nanoparticles and bromelain had a potent antioxidant effect and may be a possible therapeutic agent for many diseases in the future.

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