Attenuation Acute Effects of Passive Smoking on Pulmonary Tissues in Rats by Bee Honey

Main Article Content

Eda M.A. Alshailabi
Nura I. Al-Zail
Afaf A. Alsalhin

Abstract

The recent study's goal was to use honey to reduce the acute effects of passive smoking on rats' pulmonary tissues. A total of 28 adult male rats were categorized into four groups: (1) control group; (2) rats were given honey (100 mg/kg BW/30 days); (3) rats were exposed to the cigarette smoke for 30 days; and (4) rats were given honey (100 mg/kg BW/14 days); previously,  the rats were exposed to cigarette smoke for 30 days. The mean Wet/Dry values were significantly higher in the KAR group than in the NC rats. While the PTV rats showed a notable decline in comparison to the KAR rats, the PTV rats showed no change with the NC rats. Also, after 30 days of exposure to KAR, we observed numerous changes in the rats' gross lung lesions and more histological alterations as compared to all other rats' lungs. Furthermore, the lung tissue of the PTV rats had some histological changes and a moderate improvement in tissue structure when compared to KAR rats. In conclusion, the study demonstrated the beneficial effects of honey in lowering lung tissue damage. It is also a better treatment choice, which may lead to better results in terms of tissue damage caused by CS.

Article Details

How to Cite
[1]
Alshailabi, E.M. et al. 2024. Attenuation Acute Effects of Passive Smoking on Pulmonary Tissues in Rats by Bee Honey. Ibn AL-Haitham Journal For Pure and Applied Sciences. 37, 4 (Oct. 2024), 86–96. DOI:https://doi.org/10.30526/37.4.3955.
Section
Biology

Publication Dates

Received

2024-03-31

Accepted

2024-06-09

Published Online First

2024-10-20

References

Öberg, M.; Jaakkola, M.S.; Woodward, A.; Peruga, A.; Prüss-Ustün, A. Worldwide burden of disease from exposure to second-hand smoke: A retrospective analysis of data from 192 countries. Lancet 2011, 377(9760),139-146. https://doi.org/10.1016/s0140-6736(10)61388-8.

Yousuf, H.; Hofstra, M.; Tijssen, J.; Leenen, B.; Lindemans, J.W.; van Rossum, A.; Narula, J.; Hofstra, L. Estimated worldwide mortality attributed to secondhand tobacco smoke exposure, 1990-2016. JAMA Network Open 2020, 3(3), e201177. https://doi.org/10.1001%2Fjamanetworkopen.2020.1177.

Fildan, A.P.; Mihaltan, F.D.; Rajnoveanu, R.; Ulmeanu, R. Pulmonary effects of passive smoking among adults. Smoking prevention and cessation. Licensee InTech 2018, Chapter 1, 2-23, https://doi.org/10.5772/intechopen.77954.

Duarte, J.L.; de Faria, F.A.C.; Ceolin, D.S.; Cestari, T.M.; de Assis, G.F. Effects of passive smoke inhalation on the vocal cords of rats. Brazilian Journal of Otorhinolaryngology 2006, 2(72), 206-210. https://doi.org/10.1016/s1808-8694(15)30057-4.

Du, Y.; Cui, X.; Sidorenkov, G.; Groen, H.J.M.; Vliegenthart, R.; Heuvelmans, M.A.; Liu, S.; Oudkerk, M.; de Bock, G.H. Lung cancer occurrence attributable to passive smoking among never smokers in China: A systematic review and meta-analysis. Translational Lung Cancer Research 2020, 9(2), 204-217. https://doi.org/10.21037/tlcr.2020.02.11.

Abdel-Rahman, M.; Bauomy, A.A.; Salem, F.H.; Khalifa, M.A. Carob extract attenuates brain and lung injury in rats exposed to waterpipe smoke. Egyptian Journal of Basic and Applied Sciences 2018, 5(1), 2018, 31- 40. https://doi.org/10.1016/j.ejbas.2018.01.004.

Sandler, P.; Mastella, B.; Uchôa, D.; Jotz, G.P.; Leão, H.Z.; Cavazzola, L.T. The effects of passive tobacco smoking on the microcirculation of the abdominal wall in rats. Acta Cirurgica Brasileira 2016, 31(11), 714-719. https://doi.org/10.1590/s0102-865020160110000002.

Diniz, M.F.; Dourado, V.A.; Silva, M.E.; Pedrosa, M.L.; Bezerra, F.S.; Lima, W.G. Cigarette smoke causes changes in liver and spleen of mice newborn exposed during pregnancy. Journal of Cytology & Histology 2013, 4, 1-5. https://doi.org/10.4172/2157-7099.1000168.

Alshailabi, E.M.A.; Abdalally, O.A.; Majeed, S.F. Histopathological study on the protective effect of vitamin C against paracetamol-induced acute hepatic damage in rat. Global Libyan Journal 2021, 53, 1-15.

Pérez, E.; Rodríguez-Malaver, A.J.; Vit, P. Antioxidant capacity of Venezuelan honey in Wistar rat homogenates. Journal of Medicinal Food 2006, 9(4), 510-516. https://doi.org/10.1089/jmf.2006.9.510.

Yaman, T.; Yener, Z.; Celik, I. Histopathological and biochemical investigations of protective role of honey in rats with experimental aflatoxicosis. BMC Complementary and Alternative Medicine 2016, 16(232), 1-11. https://doi.org/10.1186/s12906-016-1217-7.

Roodi, P.A.; Moosavi, Z.; Goli, A.A.; Azizzadeh, M.; Hosseinzadeh, H. Histopathological Study of Protective Effects of Honey on Subacute Toxicity of Acrylamide-Induced Tissue Lesions in Rats’ Brain and Liver. Iranian Journal of Toxicology 2018, 12(3), 1-8.

Augustine, I.O.; Gertrude, O.N.; Loveth, A.N.; Al-Rashed, S.; Obinna, U.O.; Uchenna, E.K.; Usman, I.M.; Ogugua, E. A.; Batiha, G.E. The role of garlic and honey on nicotine-induced toxicity on the cerebellum of adult Wistar rats. Research Square 2020, 1-18. https://doi.org/10.21203/rs.3.rs-70046/v1.

Erejuwa, O.O.; Sulaiman, S.A; Ab Wahab, M.S. Honey: A novel antioxidant. Molecules (Basel, Switzerland) 2012, 17(4), 4400-4423. https://doi.org/10.3390/molecules17044400.

Kolawole, T.A.; Oyeyemi, W.A.; Adigwe, C.; Leko, B.; Udeh, C.; Dapper, D.V. Honey attenuates the detrimental effects of nicotine on testicular functions in nicotine treated Wistar rats. Nigerian journal of physiological sciences : official publication of the Physiological Society of Nigeria 2015, 30(1-2), 11-16.

Mohamed, M.; Sulaiman, S.A.; Jaafar, H.; Sirajudeen, K.N.S. Antioxidant protective effect of honey in cigarette smoke-induced testicular damage in rats. International Journal of Molecular Sciences 2011, 12(9), 5508-5521. https://doi.org/10.3390/ijms12095508.

Alshailabi, E.M.A; Al-Zail, N.I.; Darwesh, N.M. Effect of Libyan Sidr honey on thyroid gland damage induced by cigarette smoke in male rats. Journal of Pure and Applied Sciences 2023, 22(3), 88-93. https://doi.org/10.51984/jopas.v22i3.2752.

Alasil, S.M.; Omar, R.; Ismail, S.; Yusof, M.Y. Inhibition of quorum sensing-controlled virulence factors and biofilm formation in Pseudomonas aeruginosa by culture extract from novel bacterial species of Paenibacillus using a Rat model of chronic lung infection. International Journal of Bacteriology 2015, 1-16. https://doi.org/10.1155/2015/671562.

Wu, X.; Kong, Q.; Zhan, L.; Qiu, Z.; Huang, Q.; Song, X. TIPE2 ameliorates lipopolysaccharide-induced apoptosis and inflammation in acute lung injury. Inflammation research : official journal of the European Histamine Research Society 2019, 68(11), 981-92. https://doi.org/10.1007%2Fs00011-019-01280-6.

Braber, S.; Verheijden, K.A.T.; Henricks, P.A.J.; Kraneveld, A.D.; Folkerts, G. A comparison of fixation methods on lung morphology in a murine model of emphysema. American journal of physiology. Lung Cellular and Molecular Physiology 2010, 299(6), 843-851. https://doi.org/10.1152/ajplung.00192.2010.

Bancroft, J.; Stevens, A. Theory and Practice of Histological Techniques. 2nd ed. Churchill Livingstone; N.Y., 1982.

Dianat, M.; Radan, M.; Badavi, M.; Mard, VS.A.; Bayati, A.; Ahmadizadeh, M. Crocin attenuates cigarette smoke-induced lung injury and cardiac dysfunction by antioxidative effects: the role of Nrf2 antioxidant system in preventing oxidative stress. Respiratory Research 2018, 19(58), 1-20. https://doi.org/10.1186%2Fs12931-018-0766-3.

Gotts, J.E.; Abbott, J.; Fang, X.; Yanagisawa, H.; Takasaka, N.; Nishimura, S.L.; Calfee, C.S. Matthay, M.A. Cigarette Smoke Exposure Worsens Endotoxin Induced Lung Injury and Pulmonary Edema in Mice. Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco 2017, 19(9),1033–1039. https://doi.org/10.1093/ntr/ntx062.

Wawryk-Gawda, E.; Chylińska Wrzos, P.; Zarobkiewicz, M.K.; Chłapek, K.; Jodłowska Jędrych, B. Lung histomorphological alterations in rats exposed to cigarette smoke and electronic cigarette vapour. Experimental and Therapeutic Medicine 2020,19(4), 2826-2832. https://doi.org/10.3892%2Fetm.2020.8530.

Dai, Y.; Duan, K.; Huang, G.; Yang, X.; Jiang, X.; Chen, J.; Liu, P. Inhalation of electronic cigarettes slightly affects lung function and inflammation in mice. Frontiers in Toxicology 2023, 5, 1232040. DOI: https://doi.org/10.3389%2Fftox.2023.1232040.

Wiriansya, E.P.; Rahman, D.; Zuhair, M.N.; Rijal, S.; Ikram, D.; Pangnguriseng, U.A. Effects of e-cigarette vapor smoke on pulmonary alveoli in rattus norvegicus lungs. Jurnal Respirasi 2023, 9(3), 200-205. https://doi.org/10.20473/jr.v9-I.3.2023.200-205.

Ardiana, M.; Santoso, A.; Hermawan, H.O.; Nugraha, R.A.; Pikir, B.S.; Suryawan, G.I. Acute effects of cigarette smoke on endothelial nitric oxide synthase, vascular cell adhesion molecule 1 and aortic intima media thickness. F1000Research 2023, 10, 396. https://doi.org/10.12688/f1000research.28375.4.

Dogan, O.T.; Elagoz, S.; Ozsahin, S.L.; Epozturk, K.; Tuncer, E.; Akkurt, I. Pulmonary toxicity of chronic exposure to tobacco and biomass smoke in rats. Clinics (Sao Paulo) 2011, 66(6), 1081-1087. https://doi.org/10.1590%2FS1807-59322011000600027.

van der Vaart, H.; Postma, D.S.; Timens, W.; ten Hacken, N.H.T. Acute effects of cigarette smoke on inflammation and oxidative stress: A review. Thorax 2004, 59(8), 713-721. https://doi.org/10.1136/thx.2003.012468.

Morsch, A.L.B.C.; Wisniewskia, E.; Luciano, T.F.; Comina, V.H.; de Bem Silveiraa, G.; Marques, S.O.; Thirupathi, A.; Locka, P.C.S.L.; De Souza, C.T. Cigarette smoke exposure induces ROS-mediated autophagy by regulating sestrin, AMPK, and mTOR level in mice. Redox Report: Communications in Free Radical Research 2019, 24(1), 27-33. https://doi.org/10.1080/13510002.2019.1601448.

Dixon, B. Bacteria cannot resist honey. Lancet Infectious Diseases 2003, 3(2), 116. https://doi.org/10.1016/s1473-3099(03)00524-3.

Awhin, P.E.; Uzuegbu, U.E.; Ojugbeli, E.; Otomewo, L.O. Brain levels of reduced glutathione and malondialdehyde in honey-fed Wistar rats. Biosciences Biotechnology Research Asia 2021,18(3), 517-522. https://doi.org/10.13005/bbra/2936.