Synthesis and Characterization of a Magnetically Functionalized Silver-Curcumin-Chitosan Nanocomposite using Super Magnetite

Hiba Ali  Alzand; Entisar E.  Al-Abodi

doi:10.30526/zzp5mw40

Authors

Hiba Ali Alzand Department of Chemistry, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad, Baghdad, Iraq. https://orcid.org/0009-0003-4979-5254
Entisar E. Al-Abodi Department of Chemistry, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad, Baghdad, Iraq. https://orcid.org/0009-0004-8637-3939

DOI:

https://doi.org/10.30526/zzp5mw40

Keywords:

Chitosan, Green chemistry, Magnetite, Nanoparticles, Silver-curcumin

Abstract

Green synthesis of nanoparticles (NPs) has gained attention as an eco-friendly alternative to conventional methods, reducing toxicity to the environment and living organisms. This study aims to synthesize a novel nanocomposite (Mag-Chi-Cur-AgNPs) composed of silver NPs (AgNPs) prepared using curcumin (Cur) as a reducing and stabilizing agent, along with chitosan (Chit) and magnetic iron oxide (Mag). Cur-AgNPs were synthesized by dissolving Cur in dimethyl sulfoxide and deionized water, followed by the controlled addition of AgNO₃. The solution was stirred, pH-adjusted, ultrasonicated, and incubated. Chit-Cur-Ag nanocomposite was formed by dissolving nanochitosan in acetic acid, mixing it with Cur-AgNPs, then stirring, washing, centrifuging, and drying. Finally, Mag-Chit-Cur-AgNPs were obtained by coating Chit-Cur-AgNPs with magnetic iron oxide. Characterization techniques confirmed the formation of the nanocomposite. The ultraviolet-visible spectrum is used to track synthesis stages and successful formation. Fourier transform infrared spectroscopy is used to identify bond peaks, verifying molecular changes, in the field. Emission scanning electron microscope analysis measured the nanocomposite size, with particles below 100 nm. X-ray diffraction is used to determine the crystalline structure and dimensions of each component. Magnetic properties were analyzed using a vibrating sample magnetometer, demonstrating high magnetic saturation. This study successfully synthesized and characterized Mag-Chi-Cur-AgNPs using green chemistry. The results confirmed the formation and stability of the nanocomposite, showcasing its potential for future biomedical and technological applications

Author Biographies

Hiba Ali Alzand, Department of Chemistry, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad, Baghdad, Iraq.

Department of Chemistry, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad, Baghdad, Iraq.
Entisar E. Al-Abodi, Department of Chemistry, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad, Baghdad, Iraq.

Department of Chemistry, College of Education for Pure Science (Ibn Al-Haitham), University of Baghdad, Baghdad, Iraq.

References

1. Jones DL, Hodge A, Kuzyakov Y. Plant and mycorrhizal regulation of rhizodeposition. New Phytol. 2004; 163(3):459–480. https://doi.org/10.1111/j.1469-8137.2004.01130.x.

2. Yang F, Lim GP, Begum AN, Ubeda OJ, Simmons MR, Ambegaokar SS, Chen PP, Kayed R, Glabe CG, Frautschy SA, Cole GM. Curcumin inhibits formation of amyloid β oligomers and fibrils, binds plaques, and reduces amyloid in vivo. J Biol Chem. 2005; 280(7):5892–5901. https://doi.org/10.1074/jbc.M404751200.

3. Karthikeyan A, Senthil N, Min T. Nanocurcumin: A promising candidate for therapeutic applications. Front Pharmacol. 2020; 11:487. https://doi.org/10.3389/fphar.2020.00487.

4. Alavi M, Rai M, Shende S, Abd-Elsalam KA, Bajpai VK. Nanoformulations of curcumin and quercetin with silver nanoparticles for inactivation of bacteria. Cell Mol Biol. 2021; 67(5):151–156. http://dx.doi.org/10.14715/cmb/2021.67.5.21.

5. Bamal D, Singh A, Chaudhary G, Kumar M, Singh M, Rani N, Mundlia P, Sehrawat AR. Silver nanoparticles biosynthesis, characterization, antimicrobial activities, applications, cytotoxicity and safety issues: An updated review. Nanomaterials. 2021; 11(8):2086. https://doi.org/10.3390/nano11082086.

6. Atiya MA, Hassan AK, Kadhim FQ. Green synthesis of copper nanoparticles using tea leaves extract to remove ciprofloxacin (CIP) from aqueous media. Iraqi J Sci. 2021; 62(9):2832–2854. https://doi.org/10.24996/ijs.2021.62.9.1.

7. Jdayea NA, Al-Abodi EE. Biosynthesis of nanoparticles from plant extracts and their applications in medical treatments and drug delivery. Anbar J Agric Sci. 2024; 22(2):1240–1259. https://doi.org/10.32649/ajas.2024.184478.

8. Naif TA, Ahmed BJ. Synthesis, characterization and study of the effect of nanoparticles on the biological activity of new silicon polymers and their nanocomposites. Iraqi J Phys Appl Sci. 2024; 37(4): 312–322. https://doi.org/10.30526/37.4.3391.

9. Mishra B, Yadav AS, Malhotra D, Mitra T, Sinsinwar S, Radharani NNV, Rajendran M, Dey A, Shukla R. Chitosan nanoparticle-mediated delivery of curcumin suppresses tumor growth in breast cancer. Nanomaterials. 2024; 14(15):1294. https://doi.org/10.3390/nano14151294.

10. Wu Y, Zhang R, Tran HD, Kurniawan ND, Moonshi SS, Whittaker AK, Ghosh D, Nisbet DR. Chitosan nanococktails containing both ceria and superparamagnetic iron oxide nanoparticles for reactive oxygen species-related theranostics. ACS Appl Nano Mater. 2021;4(4):3604–3618. https://doi.org/10.1021/acsanm.1c00141.

11. Zaki NH, AlKaram LK, Alnuaimi M. Introduction to nanotechnology world. 1st ed. Baghdad: Adnan Library; 2024.

12. Christian P, Von der Kammer F, Baalousha M, Hofmann T. Nanoparticles: structure, properties, preparation and behaviour in environmental media. Ecotoxicology. 2008; 17(5):326–343. https://doi.org/10.1007/s10646-008-0213-1.

13. Ahmed OA, Abed AH, Al‐Saadi TM. Magnetic properties and structural analysis of Ce‐doped Mg–Cr nano‐ferrites synthesized using auto‐combustion technique. Macromol Symp. 2022; 401(1):2100311. https://doi.org/10.1002/masy.202100311.

14. Majeed AR, Al-Robaie AE. Majeed AA, Al-Robaie EA. Study the effect of adding citric acid as a fuel on the structural characteristics of the nano spinel ferrite (NiO.1CuO.2Zn0.7Fe2O4) synthesized by sol–gel auto combustion method. IHJPAS. 2015; 28(3):399-409.

15. Nowak-Jary J, Machnicka B. Comprehensive analysis of the potential toxicity of magnetic iron oxide nanoparticles for medical applications: Cellular mechanisms and systemic effects. Int J Mol Sci. 2024; 25(22):12013. https://doi.org/10.3390/ijms252212013.

16. Mohankumar S, McFarlane JR. An aqueous extract of Curcuma longa (turmeric) rhizomes stimulates insulin release and mimics insulin action on tissues involved in glucose homeostasis in vitro. Phytother Res. 2011; 25(3):396–401. https://doi.org/10.1002/ptr.3275.

17. Jaiswal S, Mishra P. Antimicrobial and antibiofilm activity of curcumin-silver nanoparticles with improved stability and selective toxicity to bacteria over mammalian cells. Med Microbiol Immunol. 2018; 207(1):39–53. https://doi.org/10.1007/s00430-017-0525-y.

18. Sadiq YM, Al-Abodi EE. Preparation and characterization of a new nano mixture, and its application as photocatalysis in self-assembly method for water treatment. AIP Conf Proc. 2019; 2190(1):032089. https://doi.org/10.1063/1.5138528.

19. Yusuf SI, Mohammad SJ, Ali MH. Scherrer and Williamson-Hall estimated particle size using XRD analysis for cast aluminum alloys. J Theor Appl Phys. 2024;Special Issue AICIS'23:17. https://doi.org/10.57647/j.jtap.2024.si-AICIS23.17.

20. Luu AT, Nguyen MT, Ta DN, Huynh CD. Fabrication and study on the structural and photocatalytic characterization of Ag doped ZnO nanostructure materials. VNUHCM J Eng Technol. 2023; 6(SI3): 80–87. https://doi.org/10.32508/stdjet.v6iSI3.1262.

21. Stati G, Rossi F, Trakoolwilaiwan T, Tung LD, Mourdikoudis S, Thanh NTK, Salvati A, Åberg C, Dawson KA. Development and characterization of curcumin-silver nanoparticles as a promising formulation to test on human pterygium-derived keratinocytes. Molecules. 2022; 27(1):282. https://doi.org/10.3390/molecules27010282.

22. Sindhu K, Rajaram A, Sreeram KJ, Rajaram R. Curcumin conjugated gold nanoparticle synthesis and its biocompatibility. RSC Adv. 2014; 4(4):1808–1818. https://doi.org/10.1039/C3RA45345F.

23. Nasrin Farsana N, Sheik Muhideen Badhusha M, Shakina J, Mohamed Roshan M. Synthesis, structural and anti-microbial application of iron oxide/chitosan/curcumin coated nanoparticles. Eur Chem Bull. 2023; 12(SI4):11371–11383. https://doi.org/10.48047/ecb/2023.12.si4.1026.

24. Mumtaz S, Ali S, Mumtaz S, Mughal TA, Tahir HM, Shakir HA. Chitosan conjugated silver nanoparticles: the versatile antibacterial agents. Polym Bull. 2022. https://doi.org/10.1007/s00289-022-04321-z.

25. Sanmugam A, Sellappan LK, Sridharan A, Manoharan S, Sairam AB, Almansour AI, Arumugam N, Karuppusamy I. Chitosan-integrated curcumin–graphene oxide/copper oxide hybrid nanocomposites for antibacterial and cytotoxicity applications. Antibiotics. 2024; 13(7):620. https://doi.org/10.3390/antibiotics13070620.

26. Taher NG, Al-Abodi EE. Anticancer activity of synthesized green silver nanoparticles against human colon cancer cell lines. Onkol Radioter. 2023; 17(11):950–960.

27. Bordbar AK, Rastegari AA, Amiri R, Ranjbakhsh E, Abbasi M, Khosropour AR. Characterization of modified magnetite nanoparticles for albumin immobilization. Biotechnol Res Int. 2014; 2014:705068. https://doi.org/10.1155/2014/705068.

28. Hartati H, Subaer S, Hasri H, Wibawa T, Hasriana H. Microstructure and antibacterial properties of chitosan-Fe3O4-AgNP nanocomposite. Nanomaterials. 2022; 12(20):3652. https://doi.org/10.3390/nano12203652.

29. Ali MN, Al-Saadi TM, Al-Faragi JK. Effect of chitosan nanoparticles loaded oxytetracycline hydrochloride on health status of common carp (Cyprinus carpio L.) infected with columnaris disease. J Phys Conf Ser. 2021; 1879(3):032075. https://doi.org/10.1088/1742-6596/1879/3/032075.

30. Abd El-Hady MM, El-Sayed Saeed S. Antibacterial properties and pH sensitive swelling of in situ formed silver–curcumin nanocomposite based chitosan hydrogel. Polymers. 2020; 12(11):2451. https://doi.org/10.3390/polym12112451.

31. Al-Saadi TM, Alsaady LJ. Preparation of silver nanoparticles by sol-gel method and study their characteristics. IHJPAS. 2015; 28(1):301–310.

32. Saleem MH, Ahmed BJ. Synthesis, characterization and study bioactivity of silver nanocomposites. Int J Pharm Res. 2020; 12(4):766–772. https://doi.org/10.31838/ijpr/134.

33. Abduljabar MA, Merza SH. Synthesis, characterization of nickel cobaltite nanoparticles and its use in removal methyl green dye from aqueous solution. IHJPAS. 2024; 37(3):264–276. https://doi.org/10.30526/37.3.3398.

34. Tran BLT, Vo TV, Chu TP, Bach DT, Nguyen TQ, Luu PHB, Nguyen TT, Nguyen TH, Le TT, Nguyen HQ. Antibacterial efficacy of low-dosage silver nanoparticle–sodium alginate–chitosan nanocomposite films against pure and clinical acne strains. RSC Adv. 2024; 14(45):33267–33280. https://doi.org/10.1039/d4ra05180g.

35. Shareef AA, Hassan ZA, Kadhim MA, Al-Mussawi AA. Antibacterial activity of silver nanoparticles synthesized by aqueous extract of Carthamus oxycantha M. Bieb. against antibiotics resistant bacteria. Baghdad Sci J. 2022; 19(3):460–470. http://dx.doi.org/10.21123/bsj.2022.19.3.0460.