Comparative Histopathological Study of Ciprofloxacin Impact on Mus Musculus Renal Cortex Infected with Multi-drug Resistance and Susceptible Uropathogenic Escherichia Coli

Omar Al-Zaidi; Estabraq A. Mahmoud; Luma Abdulhady Zwain

doi:10.30526/39.1.4204

Authors

Omar Sinan Sadiq Hussain Al-Zaidi Department of Biology, College of Education for Pure Sciences (Ibn Al-Haitham), University of Baghdad,Baghdad,Iraq. https://orcid.org/0009-0000-6110-8929
Estabraq A. Mahmoud Department of Biology, College of Education for Pure Sciences (Ibn Al-Haitham), University of Baghdad,Baghdad,Iraq. https://orcid.org/0000-0003-1729-2102
Luma Abdulhady Zwain Department of Biology, College of Education for Pure Sciences (Ibn Al-Haitham),University of Baghdad,Baghdad,Iraq. https://orcid.org/0000-0001-6636-6062

DOI:

https://doi.org/10.30526/39.1.4204

Keywords:

UPEC, Ciprofloxacin, Renal cortex, renal medulla Antibiotic resistance

Abstract

Urinary tract infections are mainly caused by uropathogenic Escherichia coli, which represent a significant global issue along with the rising of antibiotic resistance and treatment challenges. The aim of this study was to evaluate ciprofloxacin efficacy as a treatment in animal models following infection with multidrug-resistant UPEC and multidrug-susceptible UPEC and to determine the nephrotoxic effect of these antibiotics on the renal cortex. Up to 76 E. coli isolates were collected from UTI patients in Baghdad province, characterized by morphological and biochemical features, and confirmed using the Vitek-2 compact system. Mice were orally infected via gastric gavage with G33 using a bacterial load of 107 cells/ml, followed by post-infection treatment strategies with the aid of ciprofloxacin post-infection. Efficacy was determined by reduction in bacterial load, body weight, and renal cortex cross-sections. Ciprofloxacin significantly reduced the bacterial load but also caused toxicity to the kidney, such as tubular necrosis, hemorrhage, and congestion of glomeruli. This study highlights the urgent need for specialized antibiotic treatment systems to reduce drug resistance and nephrotoxicity effects. Further studies are essential to minimize renal damage.

Author Biographies

Omar Sinan Sadiq Hussain Al-Zaidi, Department of Biology, College of Education for Pure Sciences (Ibn Al-Haitham), University of Baghdad,Baghdad,Iraq.

Department of Biology, College of Education for Pure Sciences (Ibn Al-Haitham), University of Baghdad,Baghdad,Iraq.
Estabraq A. Mahmoud, Department of Biology, College of Education for Pure Sciences (Ibn Al-Haitham), University of Baghdad,Baghdad,Iraq.

Department of Biology, College of Education for Pure Sciences (Ibn Al-Haitham), University of Baghdad,Baghdad,Iraq.
Luma Abdulhady Zwain, Department of Biology, College of Education for Pure Sciences (Ibn Al-Haitham),University of Baghdad,Baghdad,Iraq.

Department of Biology, College of Education for Pure Sciences (Ibn Al-Haitham),University of Baghdad,Baghdad,Iraq.

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