Detection of Cellular and Molecular Toxicity of Aloe vera Gel Crude Extract on the Roots of the Allium cepa

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Nidhal N. Hussain
Ghaith M. Fadhil

Abstract

    The cellular and molecular toxicity effect  of Aloe Vera crude extract on meristem cells of onion  (Allium cepa)  roots were studied  . Different concentrations of this crude extract   20% ,10% ,5%   ,2% ( and 40%) were used at different periods ; 24 ,48 and 72 hour . Number of  chromosomal aberrations and the mitotic index was calculated . In addition, the genetic effects were observed by  using randomly amplified polymorphic DNA ( RAPD ) technique . The results  showed that the gel crud extract of Aloe vera  had inhibition effects on the growth of the onion roots with 10% EC50  value. Significant effects of this gel crud extract were also observed on the chromosomal aberration and mitotic index by decreasing of divided cell rates. The toxic effect of 20% concentration of gel crud extract was decreased the mitotic index under 50% , in the comparison with control sample . Chromosomal aberration were also observed in divided cells of onion roots, which exposed to different concentrations at different periods . The higher frequent type of this chromosomal aberrations , which observed  are stickiness , disturbed , bridge , c-mitosis , vagrant and star telophase . The result of RAPD technique  reveled differences in number of DNA bands and their molecular weights of the treated samples , which exposed to high concentration of crud extract in comparison with the control sample . The result of the genetic stability ratio (% GTS), also showed that Aloe vera crud extract have genetic toxicity at 40% concentration.

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How to Cite
N. Hussain, N., & M. Fadhil, G. (2017). Detection of Cellular and Molecular Toxicity of Aloe vera Gel Crude Extract on the Roots of the Allium cepa. Ibn AL-Haitham Journal For Pure and Applied Sciences, 29(1). Retrieved from https://jih.uobaghdad.edu.iq/index.php/j/article/view/66
Section
biology