Spectrophotometric Determination of Sulfathiazole Using 2,4 –dinitrophenylhydrazine as Coupling Reagent

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Israa T. Humedy
Ismael M. Ali

Abstract

A rapid, sensitive and selective spectrophotometric method was developed for determination of sulfathiazole (STHZ) in aqueous solution. The method is based on the  oxidative coupling reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) in a basic medium (pH 10.9) in the presence of  potassium periodate to produce an intense orange colour, soluble in water , stable product and absorbs at 492 nm. Beer's law was in the linear range 2.0-28.0 μg/ml of sulfathiazole, the molar absorptivity, Sandellʼs sensitivity index and detection limit were 1.1437 ×104 liter. mol-1.cm-1,0.0223 μg.cm-2 and 0.1274 μg/ml respectively. The RSD value was  0.75 - 1.12 % depending on the concentration. This method was applied successfully to the determination of sulfathiazole veterinary injection liquid solution (bio prime) with average recovery of not less than  100.38  %.

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How to Cite
Humedy, I. T., & Ali, I. M. (2017). Spectrophotometric Determination of Sulfathiazole Using 2,4 –dinitrophenylhydrazine as Coupling Reagent. Ibn AL- Haitham Journal For Pure and Applied Sciences, 29(2), 227–239. Retrieved from https://jih.uobaghdad.edu.iq/index.php/j/article/view/112
Section
Chemistry