Purification of algnic acid by Local isolate of Azotobacter Vinelandi

Main Article Content

J.I. Mahdi
M.R. Ali
H.M. Hamza

Abstract

The bacteria Azotobacter Vinelandii  was taken from a central research in Baghdad, The purification of alginic acid which produced from the bacteria by several steps starting with precipitation with isopropanol (3:1) v/v , Washing by ppt with 100ml of isopropanol       : distilled water (3:1) v/v , then the ppt was dissolved in warm distilled water and dialysis against distilled water from 24 h/s . To Complete the purification , gel filtration chromatography was conducted on sephacryl s-100 column followed by ion – exchange chromatography . Using DEAE cellulose column . The molecular Weight of purified al ginic acid was higher than that of blue dextran 2000,It was more than (2) millions Dalton .

Article Details

How to Cite
Purification of algnic acid by Local isolate of Azotobacter Vinelandi. (2017). Ibn AL-Haitham Journal For Pure and Applied Sciences, 22(2). https://jih.uobaghdad.edu.iq/index.php/j/article/view/1260
Section
Biology

How to Cite

Purification of algnic acid by Local isolate of Azotobacter Vinelandi. (2017). Ibn AL-Haitham Journal For Pure and Applied Sciences, 22(2). https://jih.uobaghdad.edu.iq/index.php/j/article/view/1260