Role of Extracted Genomic DNA on Biofilm Formation by Pseudomonas aeruginosa and Klebsiella pneumoniae in vitro

Abstract

   Bacteria form complex and highly elaborate surface adherent communities known as biofilms.Biofilm have been shown to be associated with several human diseases ,and to colonize a wide variety of medical devices . The current study focuses on contribution of extracted genomic   DNA in  biofilm formation by   P. aeruginosa and  K. pneumoniae isolates   .The percentages of Pseudomonas aeruginosa recovery from drinking water  in this study were  10%(20 positive P. aeruginosa  samples ) and K. pneumonia.,  7%(14 positive K. pneumonia samples).The results showed that all P.aeruginosa and K. pneumoniae isolates (100%) were slime producer but in different degrees by forming  of black colonies on congored agar: very  black colonies , black colonies  and red colonies  .Results  indicated that addition of extracted genomic   DNA to microtitre-plate cultures stimulate biofilm formation(either endogenous or exogenous DNA).