Evaluation the Effectiveness of Different Concentrations Phenols, Alkaloids and Terpenes Extracted from Pimpinella anisum against Phytophthora Fungi.

Main Article Content

Ahmed J. Fradi


This study did the isolation, purification, and identification of the fungus Phytophthora cinnamomi of some infected plants, including Chili pepper, cucumber, and eggplant. The green parts of Pimpinella anisum plant were grounded to a semi-powdered state. Phenols, alkaloids and terpenes were extracted from this plant, then the anti-fungal activity was evaluated at different concentrations of 5% and 10%. The percentage of radial growth inhibition of fungi with plant extracts was measured after seven days of incubation. The results showed that the terpene extract was the most effective against fungi and the alkaloid extract had the least antifungal activity. the percentage of radial growth inhibition was 97%-65%, respectively.

Article Details

How to Cite
J. Fradi, A. (2022). Evaluation the Effectiveness of Different Concentrations Phenols, Alkaloids and Terpenes Extracted from Pimpinella anisum against Phytophthora Fungi. Ibn AL- Haitham Journal For Pure and Applied Sciences, 35(2), 1–6. https://doi.org/10.30526/35.2.2746


Hossain, MB.; Brunton, NP.; Rai, DK. Effect of drying methods on the steroidal alkaloid content of potato peels, shoots and berries. Molecules .2016 ,21(4),403.

Nayak, B.; Liu, RH.; Tang, J. Effect of processing on phenolic antioxidants of fruits, vegetables, and grains—a review. Crit Rev Food Sci Nutr . 2015,55(7),887–918.

Gan, R-Y, et al.Hot air drying induces browning and enhances phenolic content and antioxidant capacity in mung bean (Vigna radiata L.) sprouts. J Food Process Preserv . 2017,41(1),e12846.

Guo, T.-T.; Zhang, J.-C.; Zhang, H.; Liu, Q.-C.; Zhao, Y.; Hou, Y.-F.; Bai, L.; Zhang, L.; Liu, X.-Q.; and Liu, X.-Y. Bioactive spirans and other constituents from the leaves of Cannabis sativa f. sativa. J. Asian Nat. Prod. Res. 2017, 19, 793–80

Ingallina, C.; Sobolev, A.P.; Circi, S.; Spano, M.; Fraschetti, C.; Filippi, A.; Di Sotto, A.; Di Giacomo, S.; Mazzoccanti, G.; Gasparrini, F. ; Cannabis sativa L.inflorescences from monoecious cultivars grown in central Italy: An untargeted chemical characterization from early flowering to ripening. Molecules, 2020, 25, 1908.

Xiao, H.; Wu, F., Shi, L., Chen, Z., Su, S., Tang, C., Wang, H., Li, Z., Li, M. ; Shi, Q. () Cinchona Alkaloid Derivative-Catalyzed Enantioselective Synthesis via a Mannich-Type Reaction and Antifungal Activity of β- Amino Esters Bearing Benzoheterocycle Moieties. Molecules, 2014,19 (4): 3955-3972.

Lopez-Meneses, A.K., Sanchez-Marinez, R.I., Quintana- Obregon, E.A., Parra-Vergara, N.V., Gonzalez-Aguilar, G. A., Lopez-Saiz, C.A. and Cortez-Rocha, M.O. In vitro Antifungal Activity of Essential oils and Major Components against Fungi Plant Pathogens. J Phytopatho. 2017,l,1-6.

Alaklabi, A.; Arif, I.A.; Ahamed, A.; Manilal, A.; Surendrakumar, R. ; Idhayadhulla, A. Larvicidal, nematicidal, antifeedant and antifungal, antioxidant activities of Mentha spicata (Lamiaceae) root extracts. Tropical Journal of Pharmaceutical Research, 2016,15(11), 2383-2390.

Enyiukwu, D.N.; Awurum, A.N.; Ononuju, C.C.; Nwaneri J.A. Significance of characterization of secondary metabolites from extracts of higher plants in plant disease management. Int. J. Adv. Agric. Res. 2014, 2,8-28

El–Mohamedy, R.S.R. ; Abdalla, A.M. Evaluation of antifungal activity of Moringa oleifera extracts as natural fungicide against some plant pathogenic fungi Invitro. Journal of Agricultural Technology. 2014, 10(4), 963-982.

Tohidi, B.; Rahimmalek, M.; Arzani, A. Essential Oil Composition, Total Phenolic, Flavonoid Contents, and Antioxidant Activity of Thymus Species Collected from Different Regions of Iran. Food Chemistry 2017, 220153–161.

Bereksi; Mohamed Senouci; Hafida Hassaïne; Chahrazed Bekhechi; Djamel Eddine Abdelouahid. Evaluation of Antibacterial Activity of Some Medicinal Plants Extracts Commonly Used in Algerian Traditional Medicine against Some Pathogenic Bacteria. Pharmacognosy Journal 2018,10(3).

Wagle, B. ; Budathoki, U. Antifungal Activities of Essential Oils and Crude Extracts of Some Aromatic Plants Against Fusarium Rot of Trichosanthes dioica. Nepal Journal of Science and Technology, 2012,13(2), 97-102.

Dhaouadi, S.; Rouissi, W.; Mougou-Hamdane, A.; Hannachi, I.; Nasraoui, B. Antifungal activity of essential oils of Origanum majorana and Lavender angustifolia against Fusarium wilt and root rot disease of melon plants. Tunisian Journal of Plant Protection, 2018, 13 (1), 39-55

Watanabe, T. Pictorial atlas of soil and seed fungi: morphologies of cultured fungi and key to species. 2nd ed. CRC Press, Boca Raton London. 2002

Burmeister, L. The Antagonistic Mechanisms Employed by Trichoderma harzianum and their Impact on the Control of the Bean Rust Fungus Uromyces appendiculatus. Ph.D. Thesis, Faculty of Science Gottfried Wilhelm Leibniz, University of Hannover. Germany. 2008

Harborne, J.B. Phytochemical methods. Chapman and Hall. New York 2nd edition.graminum. Ph.D. thesis, College of Science/Babylon University, (In Arabic). 1984

Wang, S.Y.; Wu, C.; Chu, F.; Chien, S.; Kuo, Y.; Shyur L. and Chang, S. Chemical composition and antifungal activity of essential oil isolated from Chamaecyparis formosensis Matsum. Wood. Holzforschung. 2005, 59, 295–299.

Martin, F.N.; Bull, C.T. Biological approaches for control of root pathogens of Strawberry. Phyto pathology. 2002, 92,1356-1362.

Shukla, A.; Dwivedi, S.K. Bioefficacy of plant extracts against Fusarium species causing wilt in pulses. IOSR Journal of Engineering. 2012, 2(1), 136-144.

Toaima, W.I.M.; Osman; Y.A.H. Hamed, E.S. Comparative study for the effect of spraying withblue green algae extract on some Russian dillvarieties under El-Maghara region - North Sinaiconditions. JMPS. 2017, 5(2), 112-119.

Sun, W; Shahrajabian, M.H.; Cheng, Q. Anise (Pimpinella anisum L.); a dominant spice and traditional medicinal herb for both food and medicinal purposes. Cogent Biology. 2019, 5,1673688,1-25.