The Effect of Endosulfan Pesticide in Some Biochemical Parameters of white Mice
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Abstract
The present study aimed to examine the effect of endosulfan insecticide on some molecular and biochemical parameters in white mice. Thirty mice were separated randomly into three groups for treatment with endosulfan. One group (G1) served as the control, while the other two groups received intraperitoneal injections of endosulfan G2 (3 mg/kg) and G3 (17 mg/kg) twice a week for 21 and 45 days, respectively. A biochemical study by measuring liver function parameters, including (alanine aminotransferase (ALT) and aspartate aminotransferase (AST)) and kidney function parameters, including (Blood Urea and Creatinine) and malondialdehyde (MDA), catalase activity (CAT). This study also tested DNA damage by comet assay (normal%, low%, medium%, high%). The results of renal function parameters (Blood Urea and Creatinine) were significantly increased in all treated groups after 21 and 45 days exposed to endosulfan compared with control groups. The highest value of blood urea recorded was (49.33 ±0.88 mg/dl) at 17 mg/kg for 45 days compared with the control group, and the highest value of Creatinine recorded was (1.81 ±0.13 mg/dl) at 17 mg/kg for 45 days compared with the control group. Liver function parameters (ALT and AST) significantly increased in all treated groups compared with control groups. The results of MDA, CAT enzyme, were significantly increased in all treated groups after 21 and 45 days compared with control groups. The highest value of MDA recorded was (3.93 ±0.07 μM) at 17 mg/kg for 45 days compared with the control group. Tail DNA (%) showed a significant increase at high concentrations, and the results showed a considerable increase in the severe damage of DNA in the treated group 17 mg\kg b.wt. (25.00 ±1.00)% for 45 days, compared with the control group (3.00 ±1.00) %.
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Dilna, C.; Prasanth, G. K.; Kanade, S. R. Molecular interaction studies of endosulfan with the cholinergic pathway targets–an insilico approach. Computational Toxicology, 2018, 5, 1-7.
Lubick, N. Endosulfan's exit: US EPA pesticide review leads to a ban, 2010, 1466-1466.
Darko, G.; Akoto, O.; Oppong, C. Persistent organochlorine pesticide residues in fish, sediments and water from Lake Bosomtwi, Ghana. Chemosphere, 2008, 72(1), 21-24.
Liu, L.; Bilal, M.; Duan, X.; Iqbal, H. M. Mitigation of environmental pollution by genetically engineered bacteria—current challenges and future perspectives. Science of the Total Environment, 2019, 667, 444-454.
Kim, K. H.; Kabir, E.; Jahan, S. A. Exposure to pesticides and the associated human health effects. Science of the Total Environment, 2017, 575, 525-535.
Damalas, C. A. and Koutroubas, S. D. Farmers’ exposure to pesticides: toxicity types and ways of prevention. Toxics, 2016, 4(1), 1.
Kim, S. H.; Jang, J. B.; Park, K. H.; Paik, M. K.; Jeong, S. H. Evaluation of dermal absorption rate of pesticide chlorpyrifos using in vitro rat dermal tissue model and its health risk assessment. Biomedical Science Letters, 2016, 22(4), 140-149.
Oginawati, K.; Sidhi, R.; Susetyo, S. H. Lead exposure in trader communities in industrial area of the battery recycling plant: Tangerang, Indonesia. Journal of Ecological Engineering, 2020, 21(4).
Wei, J. L.; Zhang, L. S.; Wang, J. J.; Guo F. Z.; Li, Y. B.; Zhou, X. Q.; Sun, Z. W. Endosulfan inducing blood hypercoagulability and endothelial cells apoptosis via the death receptor pathway in Wistar rats. Toxicology Research, 2015, 4(5), 1282-1288.
Zhang, L.; Wei, J.; Guo, F.; Duan, J.; Li, Y.; Shi, Z.; Sun, Z. Endosulfan activates the extrinsic coagulation pathway by inducing endothelial cell injury in rats. Environmental Science and Pollution Research, 2015, 22(20), 15722-15730.
ATSDR,A. Toxicological profile for endosulfan. Agency of Toxic Substances and Disease Registry, U.S. Department of health and human services, 2015.
Uboh, F. E.; Asuquo, E. N.; Eteng, M. U. Endosulfan-induced hepatotoxicity is route of exposure independent in rats. Toxicology and industrial health, 2011, 27(6), 483-488.
Zhao, Y. Z.; Jia, J.; Li, Y. B.; Guo, C. X.; Zhou, X. Q.; Sun, Z. W. Effects of endosulfan on the immune function of erythrocytes, and potential protection by testosterone propionate. The Journal of Toxicological Sciences, 2014, 39(5), 701-710.
Du, H.; Wang, M.; Wang, L.; Dai, H.; Wang, M.; Hong, W.; Xu, A. Reproductive toxicity of endosulfan: implication from germ cell apoptosis modulated by mitochondrial dysfunction and genotoxic response genes in Caenorhabditis elegans. Toxicological Sciences, 2015, 145(1), 118-127.
Sebastian, R. ; Raghavan, S. C. Exposure to Endosulfan can result in male infertility due to testicular atrophy and reduced sperm count. Cell death discovery, 2015, 1(1), 1-10.
Bhalli, J. A.; Ali, T.; Asi, M. R.; Khalid, Z. M.; Ceppi, M.; Khan, Q. M. DNA damage in Pakistani agricultural workers exposed to mixture of pesticides. Environmental and Molecular Mutagenesis, 2009, 50(1), 37-45.
Jan, R.; Sadique, U.; Ul Hassan, Z.; Farid, K.; Ahmad, S.; Khan, S.; Khan, H. Toxico-pathological and Reproductive Effects of Concurrent Oral Administration of Ochratoxin A and Endosulfan in Pregnant Rabbits (Oryctolagus cuniculus). Pakistan veterinary journal, 2017, 37(1).
Bouhafs, L.; Moudilou, E. N.; Exbrayat, J. M.; Lahouel, M.; Idoui, T. 2015. Protective effects of probiotic Lactobacillus plantarum BJ0021 on liver and kidney oxidative stress and apoptosis induced by endosulfan in pregnant rats. Renal failure, 2015, 37(8), 1370-1378.
Bhale, D. V.; Patil, D. S.; Mahat, R. K. Study of malondialdehyde (MDA) as a marker of oxidative stress in obese male individuals. International Journal of Recent Trends in Science and Technology, 2014,10(1), 51-52.
Ting, C. H.; Mycock, D. J.; Padayachee, K. Cold pretreatment amplifies the responses of in vitro Eucalyptus grandis shoots to cryopreparative drying. CryoLetters, 2014, 35(1), 54-62.
Guo, R.; Li, W.; Liu, B.; Li, S.; Zhang, B.; Xu, Y. Resveratrol protects vascular smooth muscle cells against high glucose-induced oxidative stress and cell proliferation in vitro. Medical science monitor basic research, 2014, 20, 82.
Guo, F. Z.; Xu, Y.; Ren, L. H.; Zhang, J.; Zhang, F.; Duan, J.; Sun, Z. W. Endosulfan induces apoptosis by activating the negative regulation pathway of cell cycle and death receptor pathway in spermatogenic cells. Toxicology Research, 2017, 6(2), 223-231.
Oyovwi, M. O.; Ben-Azu, B.; Tesi, E. P.; Oyeleke, A. A.; Uruaka, C. I.; Rotu, R. A.; Aya-Ebi, E. O. Repeated endosulfan exposure induces changes in neurochemicals, decreases ATPase transmembrane ionic-pumps, and increased oxidative/nitrosative stress in the brains of rats: Reversal by quercetin. Pesticide Biochemistry and Physiology, 2021, 175, 104833.
Hussein, M. M.; Elsadaawy, H. A.; El-Murr, A.; Ahmed, M. M.; Bedawy, A. M.; Tukur, H. A.; Saadeldin, I. M. Endosulfan toxicity in Nile tilapia (Oreochromis niloticus) and the use of lycopene as an ameliorative agent. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2019, 224,108573.
Lushchak, V. I. Environmentally induced oxidative stress in aquatic animals. Aquatic toxicology, 2011, 101(1), 13-30.
Stara, A.; Machova, J.; Velisek, J. Effect of chronic exposure to simazine on oxidative stress and antioxidant response in common carp (Cyprinus carpio L.). Environmental Toxicology and Pharmacology, 2012, 33(2), 334-343.
Stara, A.; Kristan, J.; Zuskova, E.; Velise k, J. Effect of chronic exposure to prometryne on oxidative stress and antioxidant response in common carp (Cyprinus carpio L.). Pesticide Biochemistry and Physiology, 2013, 105(1), 18-23.
Yan, J.; Wang, D.; Miao, J.; Liu, C.; Wang, Y.; Teng, M.; Zhu, W. Discrepant effects of α-endosulfan, β-endosulfan, and endosulfan sulfate on oxidative stress and energy metabolism in the livers and kidneys of mice. Chemosphere, 2018, 205, 223-233.
Tao, Y.; Pan, L.; Zhang, H.; Liu, N. Identification of genes differentially expressed in clams Ruditapes philippinarum in response to endosulfan after different exposure time. Ecotoxicology and environmental safety, 2013, 89, 108-116.
Wei, J.; Zhang, L.; Ren, L.; Zhang, J.; Liu, J.; Duan, J.; Sun, Z. Endosulfan induces cell dysfunction through cycle arrest resulting from DNA damage and DNA damage response signaling pathways. Science of The Total Environment, 2017, 589, 97-106.
Shao, B.; Zhu, L.; Dong, M.; Wang, J.; Wang, J.; Xie, H.; Zhu, S. DNA damage and oxidative stress induced by endosulfan exposure in zebrafish (Danio rerio). Ecotoxicology, 2012, 21(5), 1533-1540.
Guilherme, S.; Santos, M. A.; Barroso, C.; Gaivão, I.; Pacheco, M. Differential genotoxicity of Roundup® formulation and its constituents in blood cells of fish (Anguilla anguilla): considerations on chemical interactions and DNA damaging mechanisms. Ecotoxicology, 2012, 21(5), 1381-1390.
Khisroon, M.; Hassan, N.; Khan, A.; Farooqi, J. Assessment of DNA damage induced by endosulfan in grass carp (Ctenopharyngodon idella Valenciennes, 1844). Environmental Science and Pollution Research, 2021, 28(12), 15551-15555.