A Protective effect of (Andrographis Paniculata) on 4-Vinylcyclohexene Diepoxide Induced ovarian Toxicity in Female Albino Rats introduction
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Abstract
Abstract
Background and objective: Protective effect of (Andrographis Paniculata) on 4-Vinylcyclohexene Diepoxide.
Material and methods:A group of 55 female albino rats undergo experiment on the outcome effect of 4-vinylcyclohexene Diepoxide injection for a priod of two weeks, as well as treatment effect of methanolic leaf extract of Andrographis paniculata oral administration for four weeks. Injection concentrations (160 and 320mg/kg. B.W) also two different doses of treatment (100 and 200mg/kg. B.W) have been applied.
Results:The results on body weight and ovary weight were more evident when higher concentration dose was applied. Whereas, both induced dose were effective and no significant difference was recorded. Histological examination of ovary induced clearly the effect in various ovary tissues, and accumulation of connective tissue collagen fibers around corpus luteum where as a higher dose of treatment found to be behind sign of re-normalizing the within four weeks of treatment.
Conclusion: More detailed study proposed to findout the exact right dose for treatment and may the further study extend to include other medicinal plant that might be more efficient.
Keywords: 4-Vinylcyclohexene Diepoxide(VCD), Andrographis Paniculata Leaf, Ovarian Toxicity, Rats.
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References
Bhattacharya, S.; Haldar, P. K. Trichosanthes dioica root alleviates arsenic induced myocardial
toxicity in rats. Journal of Environmental Pathology, Toxicology and Oncology 2013, 32(3).
Kumar, G.; Singh, D.; Tali, J.A.; Dheer, D.; Shankar, R. Andrographolide: Chemical
modification and its effect on biological activities. Bioorganic chemistry 2020, 1,95,103511.
Yen, T.L.; Chen ,R.J.; Jayakumar, T.; Lu, W.J.; Hsieh, C.Y.; Hsu, M.J.; Yang, C.H.; Chang,
C.;C.; Lin, Y.K.; Lin, K.;H.; Sheu, J.;R. Andrographolide stimulates p38 mitogen-activated
protein kinase–nuclear factor erythroid-2-related factor 2–heme oxygenase 1 signaling in
primary cerebral endothelial cells for definite protection against ischemic stroke in rats.
Translational Research 2016, 1,170,57-72.
Ding, Y.; Shi, C.; Chen, L.; Ma, P.; Li, K.; Jin, J.; Zhang, Q.; Li, A. Effects of andrographolide
on postoperative cognitive dysfunction and the association with NF‑κB/MAPK pathway.
Oncology Letters 2017, 1,14(6),7367-73.
Tao, L.; Zhang, L.; Gao, R.; Jiang, F.; Cao, J.; Liu, H. Andrographolide alleviates acute brain
injury in a rat model of traumatic brain injury: possible involvement of inflammatory signaling.
Frontiers in Neuroscience 2018, 20,12,657.
Wang, L.; Cao, F.; Zhu, L.L.; Liu, P.; Shang, Y.R.; Liu, W.H.; Dong, X.; Bao, H.D.; Gong, P.;
Wang, Z.;Y. Andrographolide impairs alpha-naphthylisothiocyanate-induced cholestatic liver
injury in vivo. Journal of natural medicines 2019, 73(2),388-96.
Coon, J.T.; Ernst, E. Andrographis paniculata in the treatment of upper respiratory tract
infections: a systematic review of safety and efficacy. Planta medica. 2004, 70(04),293-8.
Chan, S.J.; Wong, W.F.; Wong, P.T.; Bian, J.S. Neuroprotective effects of andrographolide in
a rat model of permanent cerebral ischaemia. British journal of pharmacology 2010, 161(3),
-679.
Malik, Z.; Parveen, R.; Parveen, B.; Zahiruddin, S.; Khan, M.A.; Khan, A.; Massey, S. ; Ahmad,
S.; Husain, S.A. Anticancer potential of andrographolide from Andrographis paniculata (Burm.
f.) Nees and its mechanisms of action. Journal of Ethnopharmacology 2021, 272,113936.
Owoade, A.O.; Alausa, A.O.; Adetutu, A. Owoade, A.W. Protective effects of methanolic
extract of Andrographis paniculata (Burm. f.) Nees leaves against arsenic-induced damage in
rats. Bulletin of the National Research Centre 2022, 46(1),1-11.
Hu, X.; Christian, P.; Sipes, I.G.; Hoyer, P.B. Expression and redistribution of cellular Bad,
Bax, and Bcl-xL protein is associated with VCD-induced ovotoxicity in rats. Biology of
reproduction 2001, 65(5), 1489-1495.
Ameratunga, D.; Weston, G.; Osianlis, T.; Catt, J.; Vollenhoven, B. In vitro fertilisation (IVF)
with donor eggs in post-menopausal women: are there differences in pregnancy outcomes in
women with premature ovarian failure (POF) compared with women with physiological agerelated menopause?, Journal of assisted reproduction and genetics 2009, 26(9),511-514.
Shuster, L.T.; Rhodes, D.J.; Gostout, B.S.; Grossardt, B.R.; Rocca, W.A. Premature
menopause or early menopause: long-term health consequences. Maturitas 2010 65(2),161-
Sadeghi, M.R. New hopes for the treatment of primary ovarian insufficiency/premature
ovarian failure, Journal of reproduction & infertility 2013, 14(1),1.
Mahmoud, Y.I. Chronic cholestasis is associated with hypogonadism and premature ovarian
failure in adult rats (cholestasis causes ovarian hypogonadism). Ultrastructural Pathology
, 42(1), 23-31
Roshangar, L.; Hamdi, B.A.; Khaki, A.A.; Rad, J.S.; Soleimani-Rad, S. Effect of lowfrequency electromagnetic field exposure on oocyte differentiation and follicular development.
Advanced biomedical research 2014, 3.
Malahubban, M.; Alimon, A.R.; Sazili, A.Q.; Fakurazi, S.; Zakry, F.A. Phytochemical analysis
of Andrographis paniculata and Orthosiphon stamineus leaf extracts for their antibacterial and
antioxidant potential. Trop. Biomed 2013, 30(3),467-480.
Kamaruzaman, K.A.; Mat Noor, M. Reversible spermatoxic effect of Andrographis paniculata
methanol extract in Sprague Dawley rats. Malays. Appl. Biol 2012, 46, 225-232.
Juee, L.Y.; Naqishbandi, A.M. Calabash (Lagenaria siceraria) potency to ameliorate
hyperglycemia and oxidative stress in diabetes. Journal of Functional Foods 2020, 66,
,103821.
Neogy, S.; Das, S.; Mahapatra, S.K.; Mandal, N.; Roy, S. Amelioratory effect of Andrographis
paniculata Nees on liver, kidney, heart, lung and spleen during nicotine induced oxidative
stress. Environmental Toxicology and Pharmacology 2008 , 25(3), 321-328.
Tung, Y.T.; Chen, H.L.; Tsai, H.C.; Yang, S.H.; Chang, Y.C.; Chen, C.M. Therapeutic
potential of andrographolide isolated from the leaves of Andrographis paniculata Nees for treating lung adenocarcinomas. Evidence-Based Complementary and Alternative Medicine,
Rabablert, J.; Charoenteeraboon, J.; Tiewchareon, S.; Rabablert, N.; Makittithorn, W.;
Chutipanyaporn, N.; Rajchacham, S.; Junnu, V.; Yoksan, S. Activity of methanolic
Andrographis paniculate crude extract and its purified andrographolide against Japaneseencephalitis virus. Science, Engineering and Health Studies 2021, 21030007-21030007.
haref, A.Y.; Hamdi, B.A.; Alnajar, Z.A. Histopathological aspects of co-administration of
dexamethasone and diclofenac sodium on male albino rats. Zanco Journal of Medical Sciences
(Zanco J Med Sci) 2020 , 24(2), 236-245.
Muhammad, F.S.; Goode, A.K.; Kock, N.D.; Arifin, E.A.; Cline, J.M.; Adams, M.R.; Hoyer,
P.B.; Christian, P.J.; Isom, S.; Kaplan, J.R.; Appt, S.E. Effects of 4-vinylcyclohexene
diepoxide on peripubertal and adult Sprague–Dawley rats: ovarian, clinical, and pathologic
outcomes. Comparative medicine 2009, 59(1), 46-59.
Hoyer, P.B.; Devine, P.J.; Hu, X.; Thompson, K.E.; Sipes, I.G. Ovarian toxicity of 4-
vinylcyclohexene diepoxide: a mechanistic model. Toxicologic pathology 2001, 29(1), 91-99.
Özel, F.; Kiray, M.; Göker, A.; Aydemir, S.; Mıcılı, S.C. Protective effect of alpha lipoic acid
on 4-vinylcyclohexene diepoxide induced primary ovarian failure in female rats. Taiwanese
Journal of Obstetrics and Gynecology 2020, 59(2), 293-300.
Kao, S.W.; Sipes, I.G.; Hoyer, P.B. Early effects of ovotoxicity induced by 4-vinylcyclohexene
diepoxide in rats and mice. Reproductive Toxicology 1999 , 13(1),67-75.
Yunos, N.M.; Mutalip, S.S.; Jauri, M.H.; Yu, J.Q.; Huq, F. Anti-proliferative and pro-apoptotic
effects from sequenced combinations of andrographolide and cisplatin on ovarian cancer cell
lines. Anticancer research 2013, 33(10), 4365-4371.
Krishnamoorthy, P.; Sivaranjani, K.; Rajeswari, K.; Kalaiselvan, D. Effect of Andrographis
paniculata Wall. ex Nees root extract fractions on estrogen, FSH, LH, Progestrone and ovary
of female albino rats, Rattus norvegicus. 2013 .
Akowuah, G.A.; Ismail, Z.; Norhayati, I.; Sadikun, A. The effects of different extraction
solvents of varying polarities on polyphenols of Orthosiphon stamineus and evaluation of the
free radical-scavenging activity, Food chemistry 2005, 93(2), 311-317.
Sen Halicioglu, B.; Saadat, K.A.; Tuglu, M.I. The relationship of 4‐vinylcyclohexene
diepoxide toxicity with cell death, oxidative stress, and gap junctions in female rat ovaries.
Reproductive Medicine and Biology ,2021, 20(4), 543-553.
Devine, P.J.; Sipes, I.G.; Skinner, M.K.; Hoyer, P.B. Characterization of a rat in vitro ovarian
culture system to study the ovarian toxicant 4-vinylcyclohexene diepoxide. Toxicology and
applied pharmacology, 2002, 184(2),107-115.
Flaws, J.A.; Salyers, K.L.; Sipes, I.G.; Hoyer, P.B. Reduced ability of rat preantral ovarian
follicles to metabolize 4-vinyl-1-cyclohexene diepoxide in vitro. Toxicology and applied
pharmacology, 1994, 126(2), 286-294.
Flaws, J.A.; Doerr, J.K.; Sipes, I.G.; Hoyer, P.B. Destruction of preantral follicles in adult rats
by 4-vinyl-1-cyclohexene diepoxide. Reproductive Toxicology, 1994, 8(6),509-514.