Evaluation of IL-6 and IgE Levels in Iraqi Patients with Chronic Spontaneous Urticaria (CSU) in Baghdad, Iraq.

Authors

Riyam Ahmed Department of Biology, College of Science for Women, University of Baghdad, Iraq.
Talib Abdullah Hussein Department of Biology, College of Science for Women, University of Baghdad, Iraq.
Suaad Almas Brakhas Department of Immunology –Allergy Specialized Center, Baghdad / Iraq.

DOI:

https://doi.org/10.30526/36.2.3010

Keywords:

WBC, Eosinophils (EO), IL-6 and Total-IgE, CUS and Age.

Abstract

A skin condition known as chronic spontaneous urticaria (CSU), which lacks an obvious triggering factor, is characterized by recurrent transient wheals, angioedema, or both for longer than six weeks. the current study was focused to study compared and diagnosis CSU by using heamatological and immunological parameters as (White blood cell count, eosinophils (EO), IL-6 and Total-IgE, in Chronic Urticaria patients were Clinically diagnosed by dermatologists in Specialized Center of Allergy in Baghdad/Al-Rusafa with age ranged between (11-60), as well as a control group included 40 with age ranged between (11-60). The results of demographic and clinical characteristics displayed the rate of female patients was higher than male which represented by 56.3 and 43.8% respectively. The results showed there was highly significant differences between the patients and the control in WBC , Total-IgE ,IL-6 the results showed EO was higher than control but no significant difference at p ≤ 0.05. The eosinophils percentage was high in age 51-60 and 21-30 year while those with age 41-50 and 51-60 year had highest, Total-IgE level 211.7 ±38.96 IU/ml, 211.69 ±38.71IU/ml respectively but IL-6 level was highest 26.4 ±1.77 pg/ml in patients with age group 21-30 year. WBC count in age 41-50 and 51-60 year showed highest count 9.2 ±1.34 cell/cm³blood, 9.3±1.21 cell/cm³blood respectively. In same time the values of parameters were various between patients without treatment and in patients who undergoes into treatment, Finally The ROC showed the IL-6 and Total-IgE were discriminated as accurate biomarkers for CSU.

References

Gonzalez-Diaz, S.N.; Sanchez-Borges, M.; Rangel-Gonzalez, D.M.; Guzman-Avilan, R.I.; Canseco-Villarreal, J.I.; Arias-Cruz, A. Chronic Urticaria and Thyroid Pathology. World Allergy Organ. J. 2020, 13, 100101.

Choi, J.-H.; Lee, D.H.; Song, W.-J.; Choi, M.; Kwon, J.-W.; Kim, G.-W.; Kim, M.H.; Kim, M.-A.; Kim, M.-H.; Kim, B.-K. The KAAACI/KDA Evidence-Based Practice Guidelines for Chronic Spontaneous Urticaria in Korean Adults and Children: Part 2. Management of H1-Antihistamine-Refractory Chronic Urticaria. Allergy. Asthma Immunol. Res. 2020, 12, 750.

Kolkhir, P.; Church, M.K.; Altrichter, S.; Skov, P.S.; Hawro, T.; Frischbutter, S.; Metz, M.; Maurer, M. Eosinopenia, in Chronic Spontaneous Urticaria, Is Associated with High Disease Activity, Autoimmunity, and Poor Response to Treatment. J. Allergy Clin. Immunol. Pract. 2020, 8, 318–325.

Lawton, S. Identifying, Diagnosing and Managing Skin Allergies. Prim. Heal. Care 2016, 26.

RORSMAN, H. Basophilic Leucopenia in Different Forms of Urticaria. Allergy 1962, 17, 168–184.

Sutton, B.J.; Davies, A.M.; Bax, H.J.; Karagiannis, S.N. IgE Antibodies: From Structure to Function and Clinical Translation. Antibodies 2019, 8, 19.

Altrichter, S.; Zampeli, V.; Ellrich, A.; Zhang, K.; Church, M.K.; Maurer, M. IgM and IgA in Addition to IgG Autoantibodies against FcɛRIα Are Frequent and Associated with Disease Markers of Chronic Spontaneous Urticaria. Allergy 2020, 75, 3208–3215.

Kaur, S.; Bansal, Y.; Kumar, R.; Bansal, G. A Panoramic Review of IL-6: Structure, Pathophysiological Roles and Inhibitors. Bioorg. Med. Chem. 2020, 28, 115327.

Alasandagutti, M.L.; Ponnana, M.; Sivangala, R.; Thada, S.; Joshi, L.; Hussain, H.; Ansari, S.S.; Valluri, V.; Gaddam, S.L. Role of IFN-γ and IL-6 Cytokines and Their Association in Determining Susceptibility to Chronic Idiopathic Urticaria. Genet. Test. Mol. Biomarkers 2014, 18, 804–809.

Uysal, P.İ.; Hayran, Y.; Akdoğan, N.; Öktem, A.; Atılan, A.; Aksoy, G.G.; Yalçın, B. Effect of Omalizumab Therapy on Coagulation Parameters and Total Immunoglobulin E Levels in Patients with Chronic Idiopathic Urticaria and Bullous Pemphigoid. Turk Dermatoloji Derg. 2018, 12, 159.

Hatif ST, ;Hussein TA, ; Muhieldden AA. ;Evaluation of il17-a serum levels in iraqi patients with alopecia areata and alopecia universalis. Biochem. Cell Arch. 2020, 20,6419-25.

Maurer, M.; Houghton, K.; Costa, C.; Dabove, F.; Ensina, L.F.; Giménez-Arnau, A.; Guillet, G.; Konstantinou, G.N.; Labrador-Horrillo, M.; Lapeere, H. Differences in Chronic Spontaneous Urticaria between Europe and Central/South America: Results of the Multi-Center Real World AWARE Study. World Allergy Organ. J. 2018, 11, 32.

Cassano, N.; Colombo, D.; Bellia, G.; Zagni, E.; Vena, G.A. Gender-Related Differences in Chronic Urticaria. G. Ital. di Dermatologia e Venereol. Organo Uff. Soc. Ital. di Dermatologia e Sifilogr. 2015, 151, 544–552.

Hox, V.; Desai, A.; Bandara, G.; Gilfillan, A.M.; Metcalfe, D.D.; Olivera, A. Estrogen Increases the Severity of Anaphylaxis in Female Mice through Enhanced Endothelial Nitric Oxide Synthase Expression and Nitric Oxide Production. J. Allergy Clin. Immunol. 2015, 135, 729–736.

Góra, A.; Przybył, M.; Świętochowska, E.; Machura, E. Assessment of Selected Interleukins (IL-6, IL-17A, IL-18, IL-23) and Chemokines (RANTES, IP-10) in Children with Acute and Chronic Urticaria. 2022.

Jang, J.-H.; Yang, E.-M.; Lee, Y.; Ye, Y.-M.; Moon, J.; Ryu, M.S.; Park, H.-S. Increased Serum Free IgE Levels in Patients with Chronic Spontaneous Urticaria (CSU)☆. World Allergy Organ. J. 2022, 15, 100629.

Yu, Y.; Blokhuis, B.R.J.; Diks, M.A.P.; Keshavarzian, A.; Garssen, J.; Redegeld, F.A. Functional Inhibitory Siglec-6 Is Upregulated in Human Colorectal Cancer-Associated Mast Cells. Front. Immunol. 2018, 9, 2138.

Chuamanochan, M.; Kulthanan, K.; Tuchinda, P.; Chularojanamontri, L.; Nuchkull, P. Clinical Features of Chronic Urticaria in Aging Population. Asian Pacific J. allergy Immunol. 2016, 34.

Hussein TA, Brakhas SA, Dawood SM. Immunological Study in Patients with Allergic Asthma. RESEARCH JOURNAL OF PHARMACEUTICAL BIOLOGICAL AND CHEMICAL SCIENCES. 2018 , 1,9(1),421-7.

. Stone, K.D.; Prussin, C.; Metcalfe, D.D. IgE, Mast Cells, Basophils, and Eosinophils. J. Allergy Clin. Immunol. 2010, 125, S73–S80.

Ghaffar, O.; Lavigne, F.; Kamil, A.; Renzi, P.; Hamid, Q. Interleukin-6 Expression in Chronic Sinusitis: Colocalization of Gene Transcripts to Eosinophils, Macrophages, T Lymphocytes, and Mast Cells. Otolaryngol. Neck Surg. 1998, 118, 504–511.

Ertas, R.; Ozyurt, K.; Atasoy, M.; Hawro, T.; Maurer, M. The Clinical Response to Omalizumab in Chronic Spontaneous Urticaria Patients Is Linked to and Predicted by IgE Levels and Their Change. Allergy 2018, 73, 705–712.

Di Lorenzo, G.; Mansueto, P.; Pacor, M.L.; Rizzo, M.; Castello, F.; Martinelli, N.; Ditta, V.; Bianco, C. Lo; Leto-Barone, M.S.; D’Alcamo, A. Evaluation of Serum S-IgE/Total IgE Ratio in Predicting Clinical Response to Allergen-Specific Immunotherapy. J. Allergy Clin. Immunol. 2009, 123, 1103–1110.

Liu, Z.; Al-Quran, L.; Tong, J.; Cao, X. Analysis of Clinical Features and Inflammatory Related Molecules with The Disease in Acute Infectious Urticaria. 2022.

Wang, S.; Liu, Y.; Wang, J.; Zhang, J. Clinical Efficacy of Levocetirizine Combined with Ebastine in the Treatment of Chronic Urticaria and Their Effect on Serum Cytokines. Int J Clin Exp Med 2019, 12, 11675–11683.

Fujii, K.; Konishi, K.; Kanno, Y.; Ohgou, N. Acute Urticaria with Elevated Circulating Interleukin‐6 Is Resistant to Anti‐histamine Treatment. J. Dermatol. 2001, 28, 248–250.

Papadopoulos, J.; Karpouzis, A.; Tentes, J.; Kouskoukis, C. Assessment of Interleukins IL-4, IL-6, IL-8, IL-10 in Acute Urticaria. J. Clin. Med. Res. 2014, 6, 133.

Gokcek GE, Solak EÖ, Çölgeçen E. The effect of omalizumab treatment on hematological inflammatory parameters and immunoglobulin E levels in patients with chronic spontaneous urticaria. Turkish Journal of Dermatology. 2021 ,1;15(4):95.

Celik E, ; Dirican E. ; Can We Predict the Duration of Treatment Requirement by Complete Blood Count in Chronic Spontaneous Urticaria Patients Receiving Omalizumab?. Dicle Tıp Dergisi. 2020, 1;47(3),568-77.