Studying the Gene Expressions of Bla OXA-51Like and Bla OXA-23Like  in Acinetobacter baumannii

Rana Mujahid Abdullah; Arkan Adnan Mahdi

doi:10.30526/36.4.3073

Authors

Rana Mujahid Abdullah Department of Biology, College of Education for Pure Science Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq
Arkan Adnan Mahdi Department of Biology, College of Education for Pure Science Ibn Al-Haitham, University of Baghdad, Baghdad, Iraq

DOI:

https://doi.org/10.30526/36.4.3073

Keywords:

Key words:- Acinetobacter baumannii, resistance gene , gene expression

Abstract

Genotypic detection of some Antibiotics Resistant genes by using polymerase chain reaction (PCR). (20) Isolates of Acinetobacter baumannii that showed resistance to (Ceftaxim, Cefotaxim, Cefepim and Imipenim) were selected. The results showed that 20 isolates of A. baumannii possess the bla-OXA23 like gene, and that all isolates possess this gene with a percentage (100%). With molecular weight 605 bp. The current study showed that A. baumannii isolates carry 100% bla-OXA51like gene when studied with (20) isolates that are resistant to antibiotics (Imipenim Ceftazidime, Cifepime, Cifexime) that belong to this group of β-lactame with molecular weight 382 bp. Gene expression was done of the bla-_OXA23like and bla-_OXA51like. The results of gene expression for bla-_OXA23like gene show decrease the gene expression for bla-_OXA23like gene when treated by Ceftazidim, whereas the bla-_OXA51 like gene, showed increasing in gene expression when treated by Ceftazidime.

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