Detection of antibiotic resistance of the phylogenetic group E among E. coli bacteria isolated from diarrheal cases in children under five years

Main Article Content

Rana Mujahid Alshwaikh
Shahla Najim Abed Al-Azzawi

Abstract

 


From 50 stool samples collected from children with diarrhea of both sexes who visited various hospitals in Baghdad, 26 isolates of E.coli were found to belong to the phylogenetic group E. The findings revealed that the percentage of E.coli for thephylogenetic group E is (52%) , making it the dominant group among the other phylogenetic groups. The findings demonstrated that 100% of the E.coli isolates from phylogenetic group E are resistant to penicillin, and only 15% are resistant to imipenem. Multi-drug resistance (MDR) was found to be 15%, while XDR reached 85%. The results of thephylogenetic group for the remaining species of isolates in this study were group A (2/50 and by 4%), group B2 (1/50 and by 2% ),group C (12/50 and by 24%), group D (6/50 and by 12%), group F (3/50 and by 6%), group B1 by 0%, and group Clade 1 by (0%).

Article Details

How to Cite
[1]
Alshwaikh, R.M. and Abed Al-Azzawi, S.N. 2023. Detection of antibiotic resistance of the phylogenetic group E among E. coli bacteria isolated from diarrheal cases in children under five years. Ibn AL-Haitham Journal For Pure and Applied Sciences. 36, 3 (Jul. 2023), 42–50. DOI:https://doi.org/10.30526/36.3.3107.
Section
Biology

Publication Dates

References

Matsukawa, M.; Igarashi, M.; Watanabe, H.; Qin, L.; Ohnishi, M; Terajima, J.; Iyoda, S.; Morita-Ishihara, T.; Tateda, K.; Ishii, Y.; Saga, T.; Aoki, K.; Bonomo, R.A. Epidemiology and genotypic characterization of dissemination patterns of uropathogenic Escherichia coli in a community. Epidemiology and Infection 2019, 147(e148), 1–9.

Moxley, R.A.; Bargar, T.W.; Kachman, S.D.; Baker, D.R.; Francis, D.H. Intimate Attachment of Escherichia coli O157:H7 to Urinary Bladder Epithelium in the Gnotobiotic Piglet Model. Microorganisms2020, 8(2), 263.

Syahrul, F.; Wahyuni, C.U.; Notobroto, H.B.; Wasito, E.B.; Adi, A.C.; Dwirahmadi, F. (2020). Transmission media of foodborne diseases asan index prediction of diarrheagenic Escherichia coli: Study at elementary school, Surabaya, Indonesia. Int. J. Environ. Res. PublicHealth 2020, 17, 8227.

Estaleva, C.E.L.; Zimba, T.F.; Sekyere, J.O.; Govinden, U.; Chenia, H.Y.; Simonsen, G.S.; Haldorsen, B.; Essack, S.Y.; Sundsfjord, A.High prevalence of multidrug resistant ESBL- and plasmid mediated AmpC-producing clinical isolates of Escherichia coli at Maputo Central Hospital, Mozambique. BMC Infect. Dis. 2021,21, 16.

Tille, P.M. Baily and Scott’s Diagnostic Microbiology. 41st ed. Elsevier, Inc. China, 2017, 1115.

CLSI.(2021).Performance Standards for Antimicrobial Susceptibility Testing. 30th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute.

Clermont, O.; Christenson J.K.; Denamur, E.The Clermont Escherichia coli phylotyping method revisited: improvement of specificity and detection of new phylogroups. Environ. Microbiol. Rep.2013, 5, 58–65. DOI: https://doi.org/10.1111/1758-2229.12019

Clermont, O., Bonacorsi, S. and Bingen, E. Rapid and simple determination of the Escherichia coli phylogenetic group. Applied and Environmental Microbiology2000, 66(10), 4555-4558. DOI: https://doi.org/10.1128/AEM.66.10.4555-4558.2000

Clermont, O., Bonacorsi, S., and Bingen, E. Characterization of an anonymous molecular marker stronglylinked to Escherichia coli strains causing neonatal meningitis. J. Clin. Microbiol.2004,42, 1770–1772. DOI: https://doi.org/10.1128/JCM.42.4.1770-1772.2004

Lescat, M.; Clermont, O.; Woerther, P.L.; Glodt, J.; Dion, S.; Skurnik, D. Commensal Escherichia coli strains in Guiana reveal a high genetic diversity with hostdependant population structure. Environ Microbiol Rep.2012, DOI: 10.1111/j.1758-2229.2012.00374.x DOI: https://doi.org/10.1111/j.1758-2229.2012.00374.x

Clermont, O.; Lescat, M.; O’Brien, C.L.; Gordon, D.M.; Tenaillon, O.; Denamur, E. Evidence for a humanspecific Escherichia coli clone. Environ Microbiol. 2008,10, 1000–10006. DOI: https://doi.org/10.1111/j.1462-2920.2007.01520.x

Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing, M100,32nd ed.; Clinicaland Laboratory Standards Institute: Wayne, PA, USA, 2022, ISBN 978-1-68440-033-1.

AL-Imam, M.J.K. Evaluation of Lactobacillus spp. supernatant effect on gene expression of some virulence determinants in Enteropathogenic and Enterohaemorrhagic Escherichia coli from diarrheal patients. PhD thesis. College of Science, University of Baghdad, 2020.

Javadi, K.; Mohebi, S.;Motamedifar, M.; Hadi, N. Characterization and antibiotic resistance pattern of diffusely adherent Escherichia coli (DAEC), isolated from paediatric diarrhoea in Shiraz, southern Iran. New Microbes and New Infections2020, 38, 100780.

Snehaa, K.; Singh, T.; Dar, S.A.; Haque, S.; Ramachandran, V.G.; Saha, R.; Shah, D.; Das, S. Typical and atypical enteropathogenic Escherichia coli in children with acute diarrhoea: changing trend in East Delhi. Biomedical Journal2021, 44(4), 471-478.

Webale, M.K.; Guyah, B.; Wanjala, C.; Nyanga, P.L.; Webale, S.K.; Abonyo, C.; Kitungulu, N.; Kiboi, N.; Bowen, N. Phenotypic and genotypic antibiotic resistant diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in Nairobi City, Kenya. Ethiopian Journal of Health Sciences2020, 30(6).

Kurowski, K.M.; Marusinec, R.; Amato, H.K.; Garcia, C.S; Loayza, F.; Salinas, L; Trueba, G., Graham, J.P. Social and Environmental Determinants of Community-Acquired Antimicrob ial-Resistant Escherichia coli in Children Living in Semirural Communities of Quito, Ecuador. The American journal of tropical medicine and hygiene2021, 105(3), 600.

AL-Shwaikh, R. M. Antibiotics and their uses. Dar Dijla for Publishing and Distribution, Amman,2016, 112 pages.

Ejaz, H.; Younas, S.; Qamar, M.U.; Junaid, K.; Abdalla, A.E.; Abosalif, K.O.A.; Alameen, A.A.M.; Elamir, M.Y.M.; Ahmad, N.;Hamam, S.S.M. Molecular Epidemiology of Extensively Drug-Resistant mcr Encoded Colistin-Resistant Bacterial Strains Co-Expressing Multifarious -Lactamases. Antibiotics, 2021, 10, 467.

Faife, S.L.; Zimba, T.; Sekyere, J.O.; Govinden, U.; Chenia, H.Y.; Simonsen, G.S.; Sundsfjord, A.; Essack, S.Y. ß - lactam and fluoroquinolone resistance in Enterobacteriaceae from imported and locally-produced chicken in Mozambique. J. Infect. Dev. Ctries. 2020, 14, 471–478.

Pandit, R.; Awal, B.; Shrestha, S.S.; Joshi, G.; Rijal, B.P., Parajuli, N.P. Extended-Spectrum β -Lactamase (ESBL) Genotypes among Multidrug-Resistant Uropathogenic Escherichia coli Clinical Isolates from a Teaching Hospital of Nepal. Interdisciplinary Perspectives on Infectious Diseases2020. DOI: 10.1155/2020/6525826

Kutilova, I.; Medvecky, M.; Leekitcharoenphon, P.; Munk, P.; asarikova, M.; Davidova-Gerzova, L.; Jamborova, I.; Bortolaia,V.; Pamp, S.J.; Dolejska, M. Extended-Spectrum Beta-Lactamase-Producing Escherichia Coli and Antimicrobial Resistance in Municipal and Hospital Wastewaters in Czech Republic: Culture-Based and Metagenomic Approaches. Environ. Res. 2021,193, 110487.

Rodrigues, C.F. Self-medication with antibiotics in Maputo, Mozambique: Practices, rationales and relationships. Palgrave Commun. 2020,6, 1–12.

Massot, M.; Daubié, A.S.; Clermont, O.; Jaureguy, F.; Couffignal, C.; Dahbi, G.; Mora, A.; Blanco, J.; Branger, C.; Mentré, F.; Eddi, A. Phylogenetic, virulence and antibiotic resistance characteristics of commensal strain populations of Escherichia coli from community subjects in the Paris area in 2010 and evolution over 30 years. Microbiology,2016, 162(4), 642. DOI: https://doi.org/10.1099/mic.0.000242