Qualitative and Quantitative Estimation of Total Phenols in Narcissus tazettaL. Bulbs

Ola Kareem Ali; Arwa  Abdul-Kareem Tawfiq; Zainab  Yaseen Mohammed Hasan

doi:10.30526/36.4.3160

Authors

Ola Kareem Ali Department of Biology, College of Science for Women, University of Baghdad, Baghdad, Iraq
Arwa Abdul-Kareem Tawfiq Department of Biology, College of Science for Women, University of Baghdad, Baghdad, Iraq.
Zainab Yaseen Mohammed Hasan Biotechnology Research Centre, Al-Nahrain University, Baghdad, Iraq

DOI:

https://doi.org/10.30526/36.4.3160

Keywords:

Amaryllidaceae family, Narcissus tazetta, Total flavonoids, Total phenols.

Abstract

Narcissus tazetta, a member of the Amaryllidaceae family, is known to be rich in bioactive metabolites such as alkaloids, phenolics and flavonoids, which have been found in nearly every species in this family. N. tazetta cultivated in Iraq, had not previously been studied for its active components; thus, the current study used phytochemical screening and phenolic compounds estimation, both qualitatively and quantitatively. Results showed that the plant alcoholic extract was rich in alkaloids, polyphenols and flavonoids besides tannins, polysaccharides and saponins. Qualitatively; TLC and HPLC chromatogram for total phenolics and flavonoids compounds revealed the presence of Gallic acid (GA), Caffeic acid (CA), Paracumarin acid (P-c), Chlorogenic acid (Ch), Catechin (Ca), Hydroqunolinon (H) with more abundant content in Vanillic and Salycilic acids as phenolic compounds; beside, Rutin (R), Quercetin (Q), Apegenin (Ap), Luetolin (L), and Kaempferol (K) as flavonoids. Dried bulbs of N. tazetta contain total phenolic compounds reached 90 mg/g and total flavonoids in a quantity of 0.47 mg/g. Plant bulb analyzed by colorimetric reaction assay.

References

Hulcová, D.; Maříková, J.; Korábečný, J.; Hošťálková, A.; Jun, D.; Kuneš, J.; Cahlíková, L. Amaryllidaceae alkaloids from Narcissus pseudonarcissus L. cv. Dutch Master as potential drugs in treatment of Alzheimer's disease. Phytochemistry, 2019; 165, 112055.‏

Desgagné-Penix, I. Biosynthesis of alkaloids in Amaryllidaceae plants: A review. Phytochemistry Reviews, 2021; 20, 409-431.‏

Karakoyun, C.; Unver-Somer, N. Simultaneous quantitative analysis of biologically important Amaryllidaceae alkaloids in Narcissus tazetta L. subsp. tazetta by HPLC/PDA. Marmara Pharm. J., 2019; 23, 3.

Losch,, F.; Liedtke, S.; Vautz, W.; Weigend, M. Evaluation of floral volatile patterns in the genus Narcissus using gas chromatography–coupled ion mobility spectrometry. Applications in Plant Sciences, 2023; e11506.

Zarifikhosroshahi , M.; Şevket, P.; KAFKAS, E. Characterization of aroma compounds of Daffodil (Narcissus tazetta L.) ecotypes from Turkey. International Journal of Agriculture Forestry and Life Sciences 2021; 5; 101-105.

Shawky, E.; Hammoda, H.; Abou Donia, A.; Toaima, S.; Kinoshita, E.; Takayama, H. Phytochemical and biological investigation of Narcissus pseudonarcissus cultivated in Egypt. Records of Pharmaceutical and Biomedical Sciences, 2018; 2, 26-34.

Uthirapathy, D. Cardioprotection effects of diosgenin from Dioscorea bulbifera against isoproterenol-induced myocardial infarction. Drugs and Cell Therapies in Hematology, 2021; 10, 887–896.

Kalam, M.; Qayoom, I. Nargis (Narcissus tazetta L.): pharmacological properties and therapeutic uses of a rare herb of unani medicine-a review. European Journal of Pharmaceutical and Medical Research, 2021; 5, 101-105.

Rameshk, M.; Sharififar, F.; Mehrabani, M.; Pardakhty, A.; Farsinejad, A. In vitro proliferation and wound healing effects of Narcissus tazetta L bulb on primary human dermal fibroblasts. Journal of Pharmaceutical Research International, 2017; 20, 1-13. DOI: https://doi.org/10.9734/JPRI/2017/39090

Zduńska, K.; Dana, A.; Kolodziejczak, A.; Rotsztejn, H. Antioxidant properties of ferulic acid and its possible application. Skin pharmacology and physiology 2019; 31, 332-336.

Espíndola, K.M.; Ferreira, R.G.; Narvaez, L.E.M.; Silva Rosario, A.R.; Da Silva, A.M.; Silva, A.B.; Monteiro, M.C. Chemical and pharmacological aspects of caffeic acid and its activity in hepatocarcinoma. Frontiers in Oncology 2019; 541, 1-10. ‏

Al-Snafi, E. Constituents and pharmacology of Narcissus tazetta. IOSR Journal Of Pharmacy 2020,10, 44-53.

Dai J, Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010; 15; 7313–7352. DOI: https://doi.org/10.3390/molecules15107313

Cartea, E.; Francisco, M.; Soengas, P.; Velasco, P. Phenolic compounds in Brassica vegetables. Molecules, 2011; 16, 251–280. DOI: https://doi.org/10.3390/molecules16010251

Swallah, M.S.; Sun, H.; Affoh, R.; Fu, H.; Yu, H. Antioxidant potential overviews of secondary metabolites (polyphenols) in fruits. International journal of food science, 2020; 8, 9081686.

Talapatra, S.; Talapatra, B. Chemistry of Plant Natural Products: Stereochemistry, Conformation, Synthesis, Biology and Medicine, Shikimic acid pathway. Springer Berlin Heidelberg, 2015; 625–678. DOI: https://doi.org/10.1007/978-3-642-45410-3_13

Tonisi, S. Evaluation of bioactive compounds, free radical scavenging and anticancer activities of bulb extracts of Boophone disticha from Eastern Cape Province, South Africa. Saudi Journal of Biological Sciences, 2020; 27, 3559–3569.

Cannell, R. Natural Products Isolation. Human Press. Totawa, New Jersy, 1998; 165-208. DOI: https://doi.org/10.1007/978-1-59259-256-2

Pouaklin, A.; Lacroix, C.; Roux, N.; Gangheyx, G.; Deronin, F. Fungal contamination of food in Dermatology unit S.G. clean. Microbiology, 2000; 38, 4272-4273.

Marcica, M.S.; Vesna, R.; Mirza, B.; Zelijan, M. From functional food to Medicinal product: Systematic approach in analysis of polyphenolics from propolis and wine. Nutrition Journal, 2009; 33, 1-18.

Dewanto, V.; Wu, X.; Adom, K.; Liu, R. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal of Agricultural and food Chemistry, 2002; 50, 3010–3014.

Abd Mutar, M.; Hassan, Z.; Swady, N. Qualitative and quantitative evaluation of total flavonoids extracted from Mentha arvnesis Aerial Parts. Journal of Biotechnology Research Center, 2018; 12, 49-55.

Mohammed, Z. Determination of Nicotine Extracted from Eggplant and Green Pepper by HPLC. Baghdad Science Journal, 2019; 16, 0061.

Najim, R.; Hasan, Z.; AlChalabi, R. Study the antimicrobial activity of ethanolic extract of Lepidium draba on some skin infectious agents. Journal of Biotechnology Research Center 2020; 14, 10-20.

Benouchenne, D.; Bellil, I.; Bensouici, C.; Yilmaz, M.; Akkal, S.; Keskinkaya, H.; Khelifi, D. GC-MS Chemical Profile, Antioxidant Ability, Antibacterial Effect, Α-Glucosidase, Α-Amylase and Acetylcholin esterase Inhibitory Activity of Algerian Fir Essential Oil. Journal of Biological Sciences, 2022; 15, 303-310.

Talib, W.; Mahasneh, A. Antimicrobial, cytotoxicity and phytochemical screening of Jordanian plants used in traditional medicine. Molecules, 2010; 15, 1811-1824. DOI: https://doi.org/10.3390/molecules15031811

Bushra M.A.; Mahmood, O.A. Evaluating the genotoxicity enhancement of the antileukemic drug 6-mercaptopurin when combined with Iraqi Nerium oleander and Narcissus tazetta extracts in vivo. Journal of Biotechnology Research Center, 2018; 12, 92-102.

Ben-Abdallah, S.; Sefi, M.; Soudani, N.; Hamdi, A.; Bejaoui, S.; Issaoui, H. Potential antioxidant effects of Narcissus tazetta phenolic compounds against cadmium chloride-induced hepatotoxicity in Swiss albino mice. Environmental Science and Pollution Research 2021; 28, 66193-66205.

Benedec, D.; Oniga, I.; Hanganu, D.; Gheldiu, A.;, Pușcaș, C.; Silaghi-Dumitrescu, R. Sources for developing new medicinal products: biochemical investigations on alcoholic extracts obtained from aerial parts of some Romanian Amaryllidaceae species. BMC Complementary and Alternative Medicine, 2018; 18, 1-12.

Bati, A.E.; Gül, M.; Açikgöz, A.; Yarilgaç, T.; Kara, M. Assessment of Antioxidant Activity of Giant Snowdrop (Galanthus elwesii Hook) Extracts with Their Total Phenol and Flavonoid Contents. Indian Journal ofPpharmaceutical Education and Research, 2018; 52, 128-132.

Dewanto, V.; Wu, X.; Adom, K.; Liu, R. Thermal processing enhances the nutritional value of tomatoes by increasing total antioxidant activity. Journal ofAagricultural and Food Chemistry, 2002; 50, 3010–3014. DOI: https://doi.org/10.1021/jf0115589