Identification the Effective Compounds of Pumpkin Seeds and its Possibility of Application in Some Food Products

Main Article Content

Hind Mohammed Abed
Luma Khairy Hassan
Hanisah Kamilah Binti Abd Razak

Abstract

This study included the use of local pumpkin seeds of Iraqi origin, which were examined in the Seed Examination and Certification Department. It was found that they are from the plant family Cucurbitaceae Jass, and their exact scientific name is Cucuriba pepo L. This study aims to use the local Iraqi pumpkin seeds and study their effective compounds to apply these seeds to some foods. The pumpkin seeds were dried at room temperature after they were peeled, cleaned, and washed. The fiber and proximate analysis were conducted. After that, the aqueous and alcoholic extraction of the defatted and non-defatted pumpkin seeds was carried out to detect the active compounds such as tannins, glycosides, coumarins, flavonoids, resins, and saponins. For the phenols, only a positive test was given for the alcoholic extract of the defatted seeds as an indication of their presence in the oil. As for steroids and terpenes, the result is negative, and each of the extracts has a significance that is not found in the seeds. Then, the vitamins were detected in a few different proportions in pumpkin seeds, with vitamin D (26.21). As for phenols, their percentage is 14.90%, which is considered the highest result, and 13.07% for vanillin. Finally, the results of the sensory evaluation were done after cupcakes, cookies, and pudding products made from pumpkin seed flour were manufactured. The residents highly accepted those food products. Therefore, this current study aimed to find a healthy alternative to seeds to make valuable and nutritious foods. This study indicated that the manufacture of cupcakes, cookies, and pudding products from pumpkin seed flour could improve the sensory and quality properties.

Article Details

How to Cite
[1]
Mohammed Abed , H. et al. 2024. Identification the Effective Compounds of Pumpkin Seeds and its Possibility of Application in Some Food Products. Ibn AL-Haitham Journal For Pure and Applied Sciences. 37, 2 (Apr. 2024), 314–326. DOI:https://doi.org/10.30526/37.2.3336.
Section
Chemistry

Publication Dates

References

Smith, B.D. The initial domestication of Cucurbita pepo in the Americas 10,000 years ago. Science 1997, 276(5314),932-934. https://doi.org/10.1126/science.276.5314.932.

Mahmed, A.M.; Hassan, L.K.; Mohammed, B.H. Characterization the chemical and nutritional properties of Marjoram (Plant for Potential Application in Origanum vulgare) Juice industry. ‏Indian Journal of Ecology 2021, 48(17),180-184. https://www.researchgate.net/profile/Associated-Prof-Dr-LumaHassan/publication/ 358890500/links/ 621 b23459947d339eb6cdbd.pdf

Mahmed, A.M.; Mohammed, B.H.; Hassen, L.K. The role of packaging technology in the food product. In IOP Conference Series: Earth and Environmental Science. IOP 2021, 761(1),012116. https://iopscience.iop.org/article/10.1088/1755-1315/761/1/012116.

Glew, R.H.; Glew, R.S.; Chuang, L.T.; Huang, Y.S.; Millson, M.; Constans, D.; Vanderjagt, D.J. Amino acid, mineral and fatty acid content of Pumpkin seeds (Cucurbita spp) and Cyperus esculentus nuts in the Republic of Niger. Plant Foods for Human Nutrition 2006, 61(2),49-54‏. https://doi.org/10.1007/s11130-006-0010-z

Arachchige, U.S.P.R.; Wam, D.; Hbaak, L.; Mn, M.; Mawn, M. Development of extruded snacks using pumpkin flour. International Journal of Scientific and Technology Research 2019, 8(12),1564–1566. http://dr.lib.sjp.ac.lk/handle/123456789/11501

Salih, N.M.; Mohamed, A.M.; Alrawi, A.Th.; Hatem, B. Isolation and identification of Bacillus stearothermophilus and study of the inhibition effect of squeezed grape waste extract on it. Iraqi Journal of Market Research and Consumer Protection 2015, 7(1),109-125. https://www.iasj.net/iasj/journal/12/issues.

Al- Samurai, A.M.; Salih, N.M.; Alrawi, A.Th. Extraction of catechins from juiced grapes and studying their antimicrobial activity. Iraqi Agricultural Sciences Journal 2012, 43(4),112-120. https://www.iasj.net/iasj/journal/4/issues

Dhiman, A.; Babu, N.; Attri, S.; Ramachandran, P. Preparation of Pumpkin pulp and effect of different preservation methods on chemical and sensory properties during storage. Journal of Pharmacognosy and Phytochemistry 2018, 7(4),943–949. https://www.phytojournal.com/archives/2018/vol7issue4/PartP/7-3-752-678.pdf

Shaban, A.; Sahu, R.P. Pumpkin seed oil: An alternative medicine. International Journal of Pharmacognosy and Phytochemical Research 2017, 9(2(,261-273. https://doi.org/10.25258%2Fphyto.v9i2.8066

Umadevi, S.H.; Malkanthi, A. Effect of dried Pumpkin pulp and seed powder on physical, chemical and sensory properties of biscuits. International Journal of Scientific Research 2018,7(8), 32-34. https://www.researchgate.net/publication/328878957.

AOAC. “Official method of analysis”, 13th ed., Washinngton 2008. https://doi.org/10.5740/jaoacint.11-184.

Muchirah, P.N.; Wathena, R.; Muya, S.; Abubakar, L.; Ozwara, H. Makokha, A. Characterization and anti-oxidant activity of Cucurbita maxima Duchesne pulp and seed extracts. The Journal of Phytopharmacology 2018, 7(2),134-140.

https://www.researchgate.net/publication/326294127_Characterization_and_anti.

Sofowora, A. Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd., Ibadan, Nigeria 1993; pp.151-153. https://books.google.iq/books/about/Medicinal_Plants_and_Traditional_Medicin.html?id=ZdaxQwAACAAJ&redir_esc=y.

Herborne, J.B. Phytochemical methods. A guide to modern techniques of plant analysis 1973, 2,5-11.‏ file:///C:/Users/User/Downloads/Phytochemicalmethods-Harborne%20(1).pdf.

Shihata, I.M. Apharmacological study of Analagis arvensis M.D. Vet. Thesis, Cairo University 1951. https://avmj.journals.ekb.eg/article_189484_401e3888372a24023e70d7a2c55cd6e0.pdf.

Harborne, J. B. Phytochemical methods: A guide to modern techniques of plant analysis. 2nd ed., Chapman and Hall, New York 1984; pp. 1-17. file:///C:/Users/User/Downloads/Phytochemicalmethods-Harborne%20(4).pdf.

Ixtaina, V.Y.; Martínez, M.L.; Spotorno, V.; Mateo, C.M.; Maestri, D.M.; Diehl, B.W.K. Characterization of chia seed oils obtained bypressing and solvent extraction. Journal of Food Composition and Analysis 2011, 24(2),166-174.

https://www.sciencedirect.com/science/article/pii/S0889157510002851.

Stein, S.E. NIST/EPA/NIH Mass Spectral Database NIST 11) and NIST Mass Spectral Search Program Version 2.0 g. National Institute of Standards and Technology 2011. https://www.nist.gov/system/files/documents/srd/NIST1a11Ver2-0Man.pdf.

Tortora, R.; Capone, P.; De Stefano, G.; Imperatore, N.;Gerbino,N.;Donetto, S.; Monaco, V.; Caporaso, N.; Rispo, A. Metabolic syndrome in patients with coeliac disease on a gluten-free diet. Aliment Pharmacol Ther 2015, 41(4),352–359. https://doi:10.1111/apt.13062.

Hoojjat, P.; Zabik, M.E. Sugar-snap cookies prepared with wheat-navy bean-sesame seed flour blends. Cereal Chemistry 1984, 61(1),41-44. https://www.cerealsgrains.org/publications/cc/backissues/1984/Documents/chem61_41.pdf

SAS. Statistical analysis system, user's guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA 2012. https://pjbt.org/index.php/pjbt/issue/view/31.

Ardabili, A.G.; Farhoosh, R.; Khodaparast, M.H.H. Chemical composition and physicochemical properties of pumpkin seeds (Cucurbita pepo Subsp. Pepo Var. Styriaka) grown in Iran. Journal of Agricultural Science and Technology 2011, 13(7),1053-1063. https://www.sid.ir/EN/VEWSSID/J_pdf/84820110108.pdf.

Demir, I.The effects of sowing date on growth, seed yield and oil content of sunflower (Helianthus annuus L.) cultivars under rainfed conditions 2019. ‏https://hdl.handle.net/20.500.12513/4159.

Dolganyuk, V.; Belova, D.; Babich, O.; Prosekov, A.; Ivanova, S.; Katserov, D.; Sukhikh, S. Microalgae: A promising source of valuable bioproducts. Biomolecules 2020, 10(8),1153. https://doi.org/10.3390/biom10081153.

Lobo, V.; Patil, A.; Phatak, A.; Chandra, N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews 2010, 4(8),118. https://doi.org/10.4103%2F0973-7847.70902.

Syed, Q.A.; Akram, M.; Shukat, R. Nutritional and therapeutic importance of the Pumpkin seeds. Biomedical Journal of Scientific and Techincal Research 2019, 21(2),2574-1241.

http://dx.doi.org/10.26717/BJSTR.2019.21.003586.

Dowidar, M.; Ahmed, A.; Mohamed, H. The critical nutraceutical role of pumpkin seeds in human and animal health: An updated review. Zagazig Veterinary Journal 2020, 48(2),199-212.‏ https://dx.doi.org/10.21608/zvjz.2020.22530.1097.

Chuyen, H.V.; Nguyen, M.H.; Roach, P.D.; Golding, J.B.; Parks, S.E. Gac fruit (Momordica cochinchinensis Spreng.): a rich source of bioactive compounds and its potential health benefits. International Journal of Food Science & Technology 2015, 50(3),567-577. ‏https://doi.org/10.1111/ijfs.12721.

Chirumbolo, S.; Bjorklund, G.; Sboarina, A.; Vella, A. The role of vitamin D in the immune system as a pro-survival molecule. Clinical therapeutics 2017, 39(5),894-916.

https://www.sciencedirect.com/science/article/pii/S0149291817302357.

Al-Barbari, F.S.A. Chemical and technological study on some Egyptian Cucurbita cultivars. College of Agriculture, Sana'a University 2012. https://iopscience.iop.org/article/10.1088/1755.

Delouche, J.C.; Baskin, C.C. Accelerated aging techniques for predicting the relative storability of seed lots 2021.

https://scholarsjunction.msstate.edu/cgi/viewcontent.cgi?article=1009&context=seedtechpapers.

Kaur,J.; Gulati, M.; Singh, S.K.; Kuppusamy, G.; Kapoor, B.; Mishra, V.; Arshad, G.S.; Porwal, M.F.; Jha, O.; Chaitanya, N.K.; Chellappan, M.V.N.L.; Gupta, D.K.; Gupta, G.; Dua, P.K.; Khursheed, K.; Awasthi, R.; Corrie, L. Discovering multifaceted role of vanillic acid beyond flavours: Nutraceutical and therapeutic potential. Trends in Food Science & Technology 2022. https://doi.org/10.1016/j.tifs.2022.02.023.

Kim, J.K. and Park, S.U.Chlorogenic acid and its role in biological functions: an up to date. EXCLI journal 2019,18,310-316. https://www.excli.de/vol18/Park_06062019_proof.pdf.

Jnawali, P.; Kumar, V.; Tanwar, B. Celiac disease: Overview and considerations for development of gluten-free foods. Food Science and Human Wellness 2016, 5(4),169-176.

https://www.sciencedirect.com/science/article/pii/S2213453016300325.

Niland, B.; Cash, B.D. Health benefits and adverse effects of a gluten-free diet in non–celiac disease patients. Gastroenterology & hepatology 2018, 14(2),82.

https://pubmed.ncbi.nlm.nih.gov/29606920.

Ling, S.K.; Zzaman, W.; Rosidi, M.A.; Hassan, L.K.; Yang, T.A. Influence of superheated steam roasting at different temperatures and times on the color changes and fats quality of the sesame seeds and its oils. International Food Research Journal 2018, 25(6),2399-2407.

https://www.cabidigitallibrary.org/doi/pdf/10.5555/20193146912.

Abed, H.M.; Khairy, H.L. The effect of adding Pumpkin seed oil on physicochemical and sensory properties of the mayonnaise. In IOP Conference Series: Earth and Environmental Science 2023, 1214(1),012037. https://doi:10.1088/1755-1315/1214/1/012037.

Mahmed, A.M.; Mohammed, B.H.; Hassen, L.K. The role of packaging technology in the food product. In IOP Conference Series: Earth and Environmental Science 2021, 761(1),012116. https://doi:10.1088/1755-1315/761/1/012116.