The Effects of Flavonoid Extract from Capparis spinosa L. on Some Biological Parameters in Adult Male Albino Rats
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Abstract
This study aimed to explain the effect of the flavonoid extract of Cappairs Spinosa on the body weight, lipid profile, glucose, and kidney functions of male rats. We extracted the plant material using methanol at 85% in the Soxhlet apparatus for 8 hours and fractionated it with ethyl acetate. Twenty adult male rats were used in this study, which was divided into four groups (5 rats/group); the first group (T1) received 10 mg/kg. The second group (T2) received 20 mg \kg. The third group (T3) received 40 mg\kg orally for 30 days, and the 4th group (control) received orally distal water. At the end of the experiment, we collected blood samples by cardiac puncture to measure blood sugar level, lipid profile, and kidney function parameters. The levels of blood cholesterol, triglycerides, and very-low-density lipoprotein (VLDL) all went down significantly in all treated groups. On the other hand, the level of high-density lipoprotein (HDL) went up significantly compared to the control group. The blood sugar result explained a significant reduction in all treated groups compared with the control group. When kidney function parameters were measured, the amounts of urea, creatinine, and uric acid were significantly lower in animals that were given the flavonoid extract compared to those that were not. The result shows that extracts affect a significant decrease in body weight compared with control rats. These results of biological parameters explained that there were significant (P≤0.05,P≤0.01).
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Marrelli, M. Medicinal plants. Plants, 2021; 10, 7, 1355. DOI: 10.3390/plants10071355.
Zhang, H., Ma, Z. F.. Phytochemical and pharmacological properties of Capparis spinosa as a medicinal plant. Nutrients, 2018; 10, 2, 116. https://doi.org/10.3390/nu10020116.
Annaz, H.; Sane, Y.; Bitchagno, G.T.M.; Ben Bakrim, W.; Drissi, B.; Mahdi, I.; El Bouhssini, M.; Sobeh, M.. Caper (Capparis spinosa L.): an updated review on its phytochemistry, nutritional value, traditional uses, and therapeutic potential. Frontiers in Pharmacology, 2022, 13, 878749. https://doi.org/10.3389/fphar.2022.878749.
Al-Azawi, A.H.; Ghaima, K.K.; Salih, H.H. Phytochemical, antibacterial and antioxidant activities of Capparis spinosa L. Cultivated in Iraq. Bioscience Research, 2018, 15, 3, 2611-2618.
Rajhi, I.; Hernandez-Ramos, F.; Abderrabba, M.; Ben Dhia, M.T.; Ayadi, S.; Labidi, J. Antioxidant, Antifungal and Phytochemical Investigations of Capparis spinosa L. Agriculture, 2021; 11, 10, 1025. https://doi.org/10.3390/agriculture11101025.
Khanavi, M.; Ara, L.; Khavasi, N.; Hajimehdipoor, H. Capparis spinosa: a comparative study of raw and processed fruits. Journal of Medicinal Plants, 2020; 19, 73, 91-99. http://jmp.ir/article-1-2637-en.html.
Benakashani, F.; Allafchian, A.R.; Jalali, S.A.H. Biosynthesis of silver nanoparticles using Capparis spinosa L. leaf extract and their antibacterial activity. Karbala International Journal of Modern Science, 2016; 2, 4, 251-258. http://dx.doi.org/10.1016/j.kijoms.2016.08.004.
Kdimy, A.; El Yadini, M.; Guaadaoui, A.; Bourais, I.; El Hajjaji, S.; Le, H.V. Phytochemistry, Biological Activities, Therapeutic Potential, and Socio‐Economic Value of the Caper Bush (Capparis spinosa L.). Chemistry and Biodiversity, 2022; 19, 10, e202200300. DOI: 10.1002/cbdv.202200300.
Al-Badri, S.R.H.; Al Janabi, N.M.S. Estimation of the antioxidant activity of local capparis spanosa leaves. The Iraqi Journal of Agricultural Science, 2018; 49, 1, 64-70. https://doi.org/10.36103/ijas.v49i1.208.
Jawad, E.; Hasana, S.; Nassir, Z.S. Phytochemical investigation, anti-proliferative and antioxidant-activities of Iraqi Capparis spinosa L. (Family Capparidaceae) against MCF-7 human Breast. International Journal of Pharmaceutical Research, 2020; 12, 2, 1045-1055. DOI:10.31838/ijpr/2020.12.02.0158.
Saleem, H.; Khurshid, U.; Sarfraz, M.; Ahmad, I.; Alamri, A.; Anwar, S.; Alamri, A.S.; Locatelli, M.; Tartaglia, A.; Mahomoodally, M.F.; Zainal Abidin, S.A.; Ahemad, N. Investigation into the biological properties, secondary metabolites composition, and toxicity of aerial and root parts of Capparis spinosa L.: An important medicinal food plant. Food and Chemical Toxicology, 2021; 155, 112404. DOI: 10.1016/j.fct.2021.112404.
Lazeeza, S.O. Antioxidant activity of pomegranate. Iraqi Journal of Agricultural Sciences, 2021; 52, 1, 196-203. https://doi.org/10.36103/ijas.v52i1.1251.
Khadim, E.J.; Abdulrasool, A.A.; Awad, Z.J. Phytochemical investigation of alkaloids in the Iraqi Echinops heterophyllus (Compositae). Iraqi Journal of Pharmaceutical Science, 2014; 23, 1, 26-34. DOI: https://doi.org/10.31351/vol23iss1pp26-34.
Mradu, G.; Saumyakanti, S.; Sohini, M.; Arup, M. HPLC profiles of standard phenolic compounds present in medicinal plants. International Journal of Pharmacognosy and Phytochemical Research, 2012; 4, 3, 162-167.
Cary, N. Statistical analysis system, User's guide. Statistical. Version 9. SAS. Inst. Inc. USA, 2012.
Shahrajabian, M.H.; Sun, W.; Cheng, Q. Plant of the Millennium, Caper (Capparis spinosa L.), chemical composition and medicinal uses. Bulletin of the National Research Centre, 2021; 45, 1-9. https://doi.org/10.1186/s42269-021-00592-0.
Duman, Handan, et al. The antioxidant effects of capparis ovata and deferasirox in patients with thalassemia major. Mediterranean Journal of Hematology and Infectious Diseases, 2015; 112, 11, 5427. https://doi.org/10.1182/blood.V112.11.5427.5427.
Wojdyło, Aneta, et al. Polyphenol compounds and biological activity of caper (Capparis spinosa L.) flowers buds. Plants, 2019; 8, 12, 539. doi: 10.3390/plants8120539.
Moghaddasian, B.; Eradatmand Asli, D.; Alaghemand, A. Quantitative analysis of quercetin in different parts of Capparis spinosa by HPLC. Annals of Biological Research, 2012; 3, 12, 5775-5778.
Bakr, R.O.; El Bishbishy, M.H. Profile of bioactive compounds of Capparis spinosa var. aegyptiaca growing in Egypt. Revista Brasileira de Farmacognosia, 2016; 26, 514-520. https://doi.org/10.1016/j.bjp.2016.04.001.
Lemhadri, A.; Eddouks, M.; Sulpice, T.; Burcelin, R. Anti-hyperglycaemic and Anti-obesity Effects of Capparis spinosa and Chamaemelum nobile Aqueous Extracts in HFD Mice. American Journal of Pharmacology and Toxicology, 2007; 2, 3, 106-110. DOI: https://doi.org/10.3844/ajptsp.2007.106.110.
Athens, K.; Brindha, P. The antiobesity potential of Capparis spinosa flower bud extracts in 3T3-L1 adipocytes and high-fat diet-induced obese rats. Pharmaceutical Sciences Asia, 2022; 49, 5, 486-497. DOI:10.29090/psa.2022.05.22.127.
Alrawi, S.T.J.; Humide, A.O.; Al-Jeboori, M.D. Effects of Caper (Capparis spinosa) and Acetic Acid on Lipid Profile and Protein Concentration in the Serum of Albino Mice. Iraqi Journal of Science, 2021; 62, 7, 2163-2168. DOI: https://doi.org/10.24996/ijs.2021.62.7.5.
Perna, S.; Rafique, A.; Rondanelli, M.; Allehdan, S.; Riso, P.; Marino, M. Effect of caper fruit (Capparis spinosa L.) consumption on liver enzymes, lipid profile, fasting plasma glucose, and weight loss. A systematic review and a preliminary meta-analysis of randomized controlled trials. Biomedicine and Pharmacotherapy, 2023; 168, 115638. DOI: 10.1016/j.biopha.2023.115638.
Sonmezdag, A.S.; Kelebek, H.; Selli, S. Characterization of aroma‐active compounds, phenolics, and antioxidant properties in fresh and fermented capers (Capparis spinosa) by GC‐MS‐olfactometry and LC‐DAD‐ESI‐MS/MS. Journal of Food Science, 2019; 84, 9, 2449-2457. https://doi.org/10.1111/1750-3841.14777.
Rahmani, R.; Mahmoodi, M.; Karimi, M.; Hoseini, F.; Heydari, R.; Salehi, M.; Yousefi, A. Effect of hydroalcoholic extract of Capparis spinosa fruit on blood sugar and lipid profile of diabetic and normal rats. Zahedan Journal of Research in Medical Sciences, 2013; 15, 11.
Mollica, A.; Zengin, G.; Locatelli, M.; Stefanucci, A.; Mocan, A.; Macedonio, G.; Carradori, S.; Onaolapo, O.; Onaolapo, A.; Adegoke, J. Olaniyan, M.; Abdurrahman Aktumsek, A. Macedonio, G.; Novellino, E. Anti-diabetic and anti-hyperlipidemic properties of Capparis spinosa L.: in vivo and in vitro evaluation of its nutraceutical potential. Journal of Functional Foods, 2017; 35, 32-42. https://doi.org/10.1016/j.jff.2017.05.001.
Eddouks, M.; Lemhadri, A.; Michel, J.-B. Hypolipidemic activity of aqueous extract of Capparis spinosa L. in normal and diabetic rats. Journal of Ethnopharmacology, 2005; 98, 3, 345-350. DOI: 10.1016/j.jep.2005.01.053.
Vahid, H.; Rakhshandeh, H.; Ghorbani, A. Antidiabetic properties of Capparis spinosa L. and its components. Biomedicine and Pharmacotherapy, 2017; 92, 293-302. DOI: 10.1016/j.biopha.2017.05.082.
Saliem, A.H.; Hashim, S.M. Evaluation the Protective Effect of Capparis spinosa Fruits Hydroalcoholic Extract Against Nephrotoxicity Induced by Cisplatin in Rats. University of Thi-Qar Journal of Agricultural Research, 2022; 11, 2, 25-35. DOI: https://doi.org/10.54174/utjagr.v11i2.178.