The Antimicrobial Activity of Silver Nanoparticles Synthesized by Pseudomonas aeruginosa Against UTI Pathogenic Microorganisms

Mohammed R.  Gheni; Nisreen H. Odaa

doi:10.30526/37.4.3373

Authors

Mohammed R. Gheni Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. https://orcid.org/0000-0001-6871-6900
Nisreen H. Odaa Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. https://orcid.org/0000-0001-7297-1728

DOI:

https://doi.org/10.30526/37.4.3373

Keywords:

P. aeruginosa, characterization silver nanoparticles, antimicrobial activity, fourier transform infrared spectroscopy.

Abstract

Nowadays, there is an increase in interest in creating and researching silver nanoparticles (AgNPs) because of their numerous applications in many fields, especially medical fields such as burn, wound healing, dental and bone implants, antibacterial, viral, fungal, and arthropodal activities. Pseudomonas aeruginosa was isolated from burn patient identification tests by using various methods (cultural characteristics, biochemical tests, and the vitek2 system) and used to produce silver nanoparticles according to biological methods. Physical and morphological characterization was used to identify silver nanoparticles, and analysis using Fourier transform infrared spectroscopy showed the presence of 15 bands. Atomic force microscopy had an average diameter of 46.15 nm. Field emission scanning electron microscopy shows particles are aggregated into spherical to hexagonal shapes; the X-ray diffraction method results in peaks of the organic compounds in the extract that reduce the amount of silver ions and stabilize; and UV-visible spectroscopy analysis reveals a peak with maximum absorbance at 454 nm. The results showed that silver nanoparticles have antimicrobial activity against UTI pathogenic microorganisms.

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