Evaluation of Some Oxidants and Antioxidants Parameters in Iraqi Females with Celiac Disease

Farah Kamal Khalf; Nada A.   Kadhim

doi:10.30526/38.2.3459

Authors

Farah Kamal Khalf Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq. https://orcid.org/0009-0007-0143-0765
Nada A. Kadhim Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq. https://orcid.org/0000-0003-4718-3871

DOI:

https://doi.org/10.30526/38.2.3459

Keywords:

Catalase, Celiac disease, Iron deficiency anemia, Malondialdehyde, Oxidative stress

Abstract

An inherited tendency is necessary for the manifestation of celiac disease (CD), an autoimmune, gluten-sensitive, inflammatory disorder of the small intestine. Gluten, a storage protein in cereals, causes CD. An increase in oxidant compound generation, surpassing the capacity of the endogenous antioxidant system, causes oxidative stress (OS). This study aims to evaluate some oxidants and antioxidants factors in Iraqi females with CD. This hospital-based cross-sectional study at Baghdad Educational Hospital in Baghdad, Iraq, involved 100 female participants of reproductive age. Tests have been measured, such as total oxidants (TOS), total antioxidants (TAS), malondialdehyde (MDA), catalase (CAT), nitric oxide (NO), total protein (TP), and Hb. The results of the present study showed that 72 of the female patients had Hb less than 12, whereas 28 had Hb equal to or greater than 12. The anemic patient group had elevated OS indices and a low TAS. Because of the damaged small intestine, CD patients experienced an imbalance between oxidant and antioxidant parameters, which was increased with anemia.

Author Biographies

Farah Kamal Khalf, Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq.

Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq.
Nada A. Kadhim, Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq.

Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq.

References

1. King JA, Jeong J, Underwood FE, Quan J, Panaccione N, Windsor JW, Coward S, deBruyn J. Ronksley PE, Shaheen AA. Incidence of celiac disease is increasing over time: a systematic review and meta-analysis. Am J Gastroenterol. 2020; 115(4):507-525. https://doi.org/10.14309/ajg.0000000000000523.

2. Seidita A, Mansueto P, Compagnoni S, Castellucci D, Soresi M, Chiarello G, Cavallo G, De Carlo G, Nigro A, Chiavetta M, Mandreucci F, Giuliano A, Disclafani R, Carroccio A. Anemia in celiac disease: Prevalence, associated clinical and laboratory features, and persistence after gluten-free diet. J Pers Med. 2022; 12(10):1582. https://doi.org/10.3390/jpm12101582.

3. Aljada B, Zohni A, El-Matary W. The gluten-free diet for celiac disease and beyond. Nutrients. 2021; 13(11):3993. https://doi.org/10.3390/nu13113993.

4. Hamodi LE, Naji A S,Ismael S H. Factors associated with anemia in women of reproductive age in Iraqi females sample. Wiad. Lek. 2022; 75(1):164-171. https://doi.org/10.36740/WLek202201203.

5. Talarico V,Giancotti L,Mazza G A, Miniero R, Bertini M. Iron deficiency anemia in celiac disease. Nutrients. 2021; 13(5):1695. https://doi.org/10.3390/nu13051695.

6. Maluf SW, Wilhelm Filho D, Parisotto EB, Medeiros GDS, Pereira CHJ, Maraslis FT, Dornelles Schoeller CC, Rosa JSD, FrodeTS. DNA damage, oxidative stress, and inflammation in children with celiac disease. Genet Mol Biol. 2020; 43(2):e20180390. https://doi.org/10.1590/1678-4685-GMB-2018-0390.

7. Rowicka G, Czaja-Bulsa G, Chełchowska M, Riahi A, Strucińska M, Weker H, Ambroszkiewicz J. Oxidative and antioxidative status of children with celiac disease treated with a gluten free-diet. Oxid Med Cell Longev. 2018. https://doi.org/10.1155/2018/1324820.

8. Rybak A, Wierzbicka A, Socha P, Stolarczyk A, Cukrowska B, Obrycki L, Wawer Z, Janas R, Oralewska B, Szaflarska-Poplawska A, Iwańczak B, Cyrta-Jarocka E, Grzybowska-Chlebowczyk U, Cichy W, Czaja-Bulsa G, Socha J. Atherosclerotic risk factors in children with celiac disease. Gastroenterol Res Pract. 2020; 2020:6138243. https://doi.org/10.1155/2020/6138243.

9. Stojiljkovic V, Todorovic A, Pejic S, Kasapovic J, Saicic ZS. Radlovic N, Pajovic S B. Antioxidant status and lipid peroxidation in small intestinal mucosa of children with celiac disease. Clin Biochem. 2009; 42(13-14):1431-1437. https://doi.org/10.1016/j.clinbiochem.2009.06.009.

10. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005; 38(12):1103-1111. https://doi.org/10.1016/j.clinbiochem.2005.08.008.

11. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004; 37(2):112-119. https://doi.org/10.1016/j.clinbiochem.2003.10.014.

12. Hadwan MH, Khabt H. Simple spectrophotometric method for analysis of serum catalase activity. J Clin Diagn Res. 2018; 12(9):13-16. https://doi.org/10.7860/JCDR/2018/35014.12011.

13. Navarro-Gonzalvez J A, García-Benayas C, Arenas J. Semiautomated measurement of nitrate in biological fluids. Clin Chem. 1998; 44(3):679-681. https://doi.org/10.1093/clinchem/44.3.679.

14. Janssen-Heininger YM, Mossman BT, Heintz NH, Forman HJ, Kalyanaraman B, Finkel T, Stamler JS, Rhee SG, Vliet A. Redox-based regulation of signal transduction: principles, pitfalls, and promises. Free Radic Biol Med. 2008; 45 (1):1-17. https://doi.org/10.1016/j.freeradbiomed.2008.03.011.

15. Veres-Szekely A, Bernath M, Pap D, Rokonay R, Szebeni B, TakácsI. M, Lippai R, Cseh A, Szabo AJ, Vannay A. PARK7 diminishes oxidative stress-induced mucosal damage in celiac disease. Oxid Med Cell Longev. 2020; 2020:4787202. https://doi.org/10.1155/2020/4787202.

16. Montoro-Huguet M A, Santolaria-Piedrafita S, Canamares-Orbis P, Garcia-Erce J A. Iron deficiency in celiac disease: prevalence, health impact, and clinical management. Nutrients. 2021; 13(10):3437. https://doi.org/10.3390/nu13103437.

17. Bhattacharyya A, Chattopadhyay R, Mitra S, Crowe S E. Oxidative stress: an essential factor in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev. 2014; 94(2):329-354. https://doi.org/ 10.1152/physrev.00040.2012.

18. Piazza S, Colombo F, Bani C, Fumagalli M, Vincentini O, Sangiovanni E, Martinelli G,Biella S,Silano M,Restani P,et al. Evaluation of the potential anti-inflammatory activity of black rice in the framework of celiac disease. Foods. 2022; 12(1):63. https://doi.org/10.3390/foods12010063.

19. Pham-Huy L A,He H, Pham-Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci. 2008; 4(2):89-96. https://doi.org/10.59566/IJBS.2008.4089.

20. Moretti S, Mrakic-Sposta S, Roncoroni L, Vezzoli A, DellanoceC, Monguzzi E, Branchi F, Ferretti F, Lombardo V, Doneda L. Oxidative stress as a biomarker for monitoring treated celiac disease. Clin Transl Gastroenterol. 2018; 9(6):157. https://doi.org/10.1038/s41424-018-0031-6.

21. Kashyap P, Shamim M Z, gambi Singha K L. Development of antioxidant rich dietary supplement for celiac disease. J Med Pharm Allied Sci. 2022; 11(3):4862. https://doi.org/10.55522/jmpas.V11I3.2669.

22. Al-Mashhadani WM. Celiac disease is a member of oxidative stress syndrome. Iraqi J Comm Med. 2009; 22(2):88-91.

23. Halliwell B. Lipid peroxidation: a radical chain reaction. Free radicals in biology and medicine. 1989, p.188-267.

24. Ferretti G, Bacchetti T, Masciangelo S, Saturni L. Celiac disease, inflammation and oxidative damage: a nutrigenetic approach. Nutrients. 2012; 4(4):243-257. https://doi.org/10.3390/nu4040243.

25. Piątek-Guziewicz A, Dąbek A, Przybylska‑Felus M, Zagrodzki P, Mach T, Zwolinska‑Wcisło M. Oral vitamin E supplementation in reducing nitrosative stress in adults treated for celiac disease. Pol Arch Intern Med. 2020; 130(7-8):711-713. https://doi.org/10.20452/pamw.15369.

26. Piątek-Guziewicz A, Zagrodzki P, Paśko P, Krosniak M, Ptak-Belowska A, Przybylska-Feluś M, Mach T, Zwolinska-Wcisło M. Alterations in serum levels of selected markers of oxidative imbalance in adult celiac patients with extraintestinal manifestations: pilot study. Pol Arch Intern Med. 2017; 127(7-8):532-539. https://doi.org/10.20452/pamw.4020.

27. Kodydkova J, Vavrov L, Kocik M, Zak A. Human catalase, its polymorphisms, regulation and changes of its activity in different diseases. Folia Biol. 2014; 60(4):153-167. https://doi.org/10.14712/fb2014060040153.

28. Cabello-Verrugio C, Ruiz-Ortega M, Mosqueira M, Simon F. Oxidative stress in disease and aging: mechanisms and therapies. Oxid Med Cell Longev. 2016; 2016:8786564. https://doi.org/ 10.1155/2016/8786564.

29. Rovaris EM, Faraco A, Simes PW, Michels M, Madeira, K, de Pra, SDT, Vieira A, Burger H, Sonai B, Streck E, Dal-Pizzol F. Oxidative stress and inflammatory parameters in adult patients presenting with celiac disease. RASBRAN. 2017; 8(1):64-69. http://dx.doi.org/10.37111/braspenj.2017.32.2.11.

30. Glemziene I, Kazėnaite E, Kalibatiene D, Luksiene A. Enteropathies and oxidative stress. Acta Medica Lituanica. 2006; 13(4):232-235.

31. Odetti P, Valentini S, Aragno I, Garibaldi S, Pronzato M A, Rolandi E, Barreca T. Oxidative stress in subjects affected by celiac disease. Free Radic Res. 1998; 29(1):17-24. https://doi.org/10.1080/10715769800300031.