Morphological and Molecular studies of Acanthobra marmid Heckel, 1843 (Piscies, Cypriniformes, Leuciscidae) from the Middle of Iraq

Hind Dyia Hadi; Abed Hassan Baraaj; Atheer Hussein Ali

doi:10.30526/38.1.3555

Authors

Hind Dyia Hadi Iraq Natural History Research Center &Museum, University of Baghdad, Baghdad, Iraq https://orcid.org/0000-0002-0779-0194
Abed Hassan Baraaj Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq. https://orcid.org/0000-0001-8842-8519
Atheer Hussein Ali Department of Fisheries and Marine Resources, College of Agriculture, University of Basrah, Basrah, Iraq. https://orcid.org/0000-0002-2541-968X

DOI:

https://doi.org/10.30526/38.1.3555

Keywords:

Cytochrome b, Fishes, Leuciscidae, Morphometric, Meristic character, Tigris River

Abstract

Leuciscidae species are the abundant and widely distributed fish species in Iraq's inland waters. They are complex species, and morphology makes them difficult to identify. Molecular analysis achieved and confirmed the morphological characters. Twenty specimens of Acanthobrama marmid were collected from two localities at Tigris River, in the middle of Iraq; 15 specimens from the Al-Zubaydia sub-district and five specimens from Al-Tharthar Lake. We used the mitochondrial DNA cytochrome b (cytb) gene to sequence the DNA of A. marmid. The following analysis are compared the sequences with those of other fish genera and species found in the Gene Bank. The barcoding result (DNA sequencing) in fishes found in the same family (Leuciscidae) showed that it fit with A. marmid. We conclude, the results of the current study, by using the cytochrome b (cytb) gene, are a successful tool and confirmed the morphological characters for accurate identification of A. marmid.

Author Biographies

Hind Dyia Hadi, Iraq Natural History Research Center &Museum, University of Baghdad, Baghdad, Iraq

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Abed Hassan Baraaj, Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.

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Atheer Hussein Ali, Department of Fisheries and Marine Resources, College of Agriculture, University of Basrah, Basrah, Iraq.

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