Spectrophotometric Determination of Carbamazepine Via Oxidative Coupling Reaction with 2,4-dinitrophenyl hydrazine
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An accurate and sensitive spectrophotometric method has been developed for the determination of carbamazepine (CRN.) in pure and dosage forms. The method is based on the oxidation of 2,4-dinitrophenylhydrazine (2,4-DNPHz) by potassium periodate than coupling with carbamazepine (CRN.) in alkaline medium to form a stable yellowish brown colored water-soluble dye with a maximum absorption at 485 nm. The variables that affect the completion of reaction have been carefully optimized. Beerâ€™s law is obeyed over the concentration range of (4-50 Î¼g.mL-1) with molar absorptivity of (6.7335Ã—103 L.mol-1.cm1). The limit of detection was (0.1052 Î¼g.mL-1) and Sandellâ€™s sensitivity value was 0.0350 Î¼g.cm-2.The proposed method has been applied successfully to the determination of carbamazepine in pharmaceutical preparation.