Estimated of Structural, Morphology and Antibacterial properties: Plant-Mediated Synthesis of AgO, NiO, and NiO - AgO Nanoparticles Using Allium cepa extract
DOI:
https://doi.org/10.30526/39.4.4446Keywords:
Allium cepa extract, Biomedical application, AgO NPs, NiO NPs NiO-AgO NCs, Green synthesisAbstract
Allium cepa extract was used to prepare AgO NPs, NiO NPs, NiO-AgO NCs by plant-mediated synthesis, all characteristics of these nanoparticles and antibacterial properties have been studied. XRD analysis confirmed the crystalline nature of the synthesized nanoparticles. The average crystalline size was 14.75nm, 38nm and 36.4 nm for AgO NPs, NiO NPs and NiO-AgO NCs respectively. NiO NPs were hexagonal, while AgO NPs were monoclinic phase. The NiO-AgO NCs represents a hybrid structure composed of NiO NPs and AgO NPs phase. EDX analysis verified that all samples contained the elements that were present (O, Ni, and Ag). UV-Vis was used to calculate the AgO, NiO, and NiO-AgO energy gaps are 3.11 eV, 3.08 eV and 3.38 eV respectively. Additionally, From FTIR spectroscopy, the investigation found that the extract from Allium cepa included functional groups that serve as agents that reducing and capping, the spectrum verifies existence of AgO and NiO and combination of AgO and NiO with adsorbed surface groups such carbonates and water Furthermore, from zeta potential, AgO NPs, NiO NPs and NiO-AgO NCs showed great stability at -29.1 mV, -22.6 mV, and -21.3 mV respectively. The study revealed that the material demonstrates greater antibacterial activity against Gram negative bacteria compared to Gram-positive ones. The NiO-AgO NCs showed the maximum inhibition values against E. coli and Staphylococcus aureus at a concentration of 75%, measuring about 28 mm and 27 mm, respectively
References
1. Aruna V, Mundru K, Mokkapati V, Dhulipalla BS. Metal-based nanosystems and the evaluation of their antimicrobial activity. In: Antimicrobial Nanosystems. Elsevier; 2023.149–190. https://doi.org/10.1016/b978-0-323-91156-6.00009-9
2. Yudishter, Shams R, Dash KK. Polysaccharide nanoparticles as building blocks for food processing applications: a comprehensive review. Food Sci Biotechnol. 2025;34(3):527–546. https://doi.org/10.1007/s10068-024-01695-w
3. Devanesan S, AlSalhi MS. Green synthesis of silver nanoparticles using the flower extract of Abelmoschus esculentus for cytotoxicity and antimicrobial studies. Int J Nanomedicine. 2021;16(1):3343–3356. https://doi.org/10.2147/ijn.s307676
4. Patil SP. Calotropis gigantea assisted green synthesis of nanomaterials and their applications: a review. Beni-Suef Univ J Basic Appl Sci. 2020;9(1):14. https://doi.org/10.1186/s43088-020-0036-6
5. Alzidi AF, Nasir EM. Green synthesis and characterization of ZnO, CuO and ZnO@CuO nanoparticles for antibacterial activity. Ibn Al-Haitham J Pure Appl Sci. 2025;38(4):169–179. https://doi.org/10.30526/38.4.4141
6. Alias MFА, Abdulwahed SH, Hasan ZM. Characterization silver nanoparticles synthesized from Capsicum annuum peels extract using green method as anticancer agent. In: AIP Conference Proceedings. 2025;3190(1):040001. https://doi.org/10.1063/5.0292766
7. Gupta D, Boora A, Thakur A, Gupta TK. Green and sustainable synthesis of nanomaterials: recent advancements and limitations. Environ Res. 2023;231(1): 116316. https://doi.org/10.1016/j.envres.2023.116316
8. Osman AI, Zhang Y, Farghali M, Rashwan AK, Eltaweil AS, Abd El-Monaem EM, Elgarahy AM, Al-Muhtaseb AH, Rooney DW, Yap PS. Synthesis of green nanoparticles for energy, biomedical, environmental, agricultural, and food applications: A review. Environ Chem Lett. 2024;22(2):841–887. https://doi.org/10.1007/s10311-023-01682-3
9. Ayub A, Wani AK, Malik SM, Ayub M, Singh R, Chopra C, Verma A, Khan R, Sharma P.
Green nanoscience for healthcare: advancing biomedical innovation through eco-synthesized nanoparticles. Biotechnol Rep. 2025;45(1):e00913. https://doi.org/10.1016/j.btre.2025.e00913
10. Ying S, Guan Z, Ofoegbu PC, Clubb P, Rico C, He F. Green synthesis of nanoparticles: current developments and limitations. Environ Technol Innov. 2022;26(1):102336. https://doi.org/10.1016/j.eti.2022.102336
11. Radulescu DM, Surdu VA, Ficai A, Ficai D, Grumezescu AM, Andronescu E. Green synthesis of metal and metal oxide nanoparticles: a review of the principles and biomedical applications. Int J Mol Sci. 2023;24(20):15397. https://doi.org/10.3390/ijms242015397
12. Haritha V, Gowri S, Janarthanan B, Faiyazuddin M, Karthikeyan C, Sharmila S. Biogenic synthesis of nickel oxide nanoparticles using Averrhoa bilimbi and investigation of its antibacterial, antidiabetic and cytotoxic properties. Inorg Chem Commun. 2022;144(1):109930. https://doi.org/10.1016/j.inoche.2022.109930
13. Anandan B, Rajendran V. Morphological and size effects of NiO nanoparticles via solvothermal process and their optical properties. Mater Sci Semicond Process. 2011;14(1):43–47. https://doi.org/10.1016/j.mssp.2011.01.001
14. Ahmad B, Khan MI, Naeem MA, Alhodaib A, Fatima M, Amami M. Green synthesis of NiO nanoparticles using Aloe vera gel extract and evaluation of antimicrobial activity. Mater Chem Phys. 2022;288(1):126363. https://doi.org/10.1016/j.matchemphys.2022.126363
15. Rafique MA, Kiran S, Javed S, Ahmad I, Yousaf S, Iqbal N. Green synthesis of nickel oxide nanoparticles using Allium cepa peels for degradation of Congo red dye: an environmental remedial approach. Water Sci Technol. 2021;84(10–11):2793–2804. https://doi.org/10.2166/wst.2021.237
16. Shanan ZJ, Shanshool SK. Nickel oxide nanoparticles: synthesis and evaluation for antimicrobial efficacy. Int J Nanosci. 2023;22(2):2350008. https://doi.org/10.1142/s0219581x23500084
17. More PR, Pandit S, De Filippis A, Franci G, Mijakovic I, Galdiero M. Silver nanoparticles: bactericidal and mechanistic approach against drug-resistant pathogens. Microorganisms. 2023;11(2):369. https://doi.org/10.3390/microorganisms11020369
18. Xu L, Wang YY, Huang J, Chen CY, Wang ZX, Xie H. Silver nanoparticles: synthesis, medical applications and biosafety. Theranostics. 2020;10(20):8996. https://doi.org/10.7150/thno.45413
19. Abdalameer NK, Khalaph KA, Ali EM. Ag/AgO nanoparticles: green synthesis and investigation of their bacterial inhibition effects. Mater Today Proc. 2021;45(1):5788–5792. https://doi.org/10.1016/j.matpr.2021.03.166
20. Bruna T, Maldonado-Bravo F, Jara P, Caro N. Silver nanoparticles and their antibacterial applications. Int J Mol Sci. 2021;22(13):7202. https://doi.org/10.3390/ijms22137202
21. Govindhan M. Novel synthesis of neem extract NiO nanoparticles prepared by photon-induced method for supercapacitor applications. Ceram Int. 2025;51:in press. https://doi.org/10.1016/j.ceramint.2025.07.373
22. Chinnaiah K, Kannan K, Krishnamoorthi R, Palko N, Gurushankar K. Nanostructured Ag/NiO composites for supercapacitor and antibacterial applications and in silico theoretical investigation. J Phys Chem Solids. 2024;184(1):111730. https://doi.org/10.1016/j.jpcs.2023.111730
23. Shad S, Kareem L, Uddin ST, Qazi NUS, Ibrar A, Iqbal M. Facile synthesis of Ag-doped NiO nanoparticles for efficient photocatalytic degradation of ciprofloxacin and evaluation of biological activities. Sep Sci Technol. 2025;60(1):1–20. https://doi.org/10.1080/01496395.2025.2566191
24. Ravishankar TN, Ananda A, Shilpa BM, Adarsh JR. Eco-friendly synthesis of NiO and Ag/NiO nanoparticles: applications in photocatalytic and antibacterial activities. R Soc Open Sci. 2025;12(2):241733. https://doi.org/10.1098/rsos.241733
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