Improvement the Growth of Nigella sativa L. Affected by Hydrogen Peroxide by Foliar Spraying with Glutamic Acid
Keywords:
Black cumin , oxidative stress , hydrogen peroxide , glutamic acid .Abstract
Glay pots experiments were carried out in the botanical garden of Biology Department/ College of Education for Pure Science Ibn AL-Haitham / Baghdad University for the growing season 2014-2015 , to evaluate the effect of foliar spraying of hydrogen peroxide ( H2O2) and glutamic acid and their interaction on some growth parameters and yield components of black cumin plant . The experiment included the following factors :- 1- Four concentrations of hydrogen peroxide (0 , 5 , 10 , 20 ) mM.L-1 . 2- Three concentrations of glutamic acid ( 0 , 50 , 100 ) mg.L-1 . The experiment was designed according to completely randomized design (CRD) with three replications , Results revealed that foliar spraying with hydrogen peroxide caused a significant decrease in the growth parameters while the spraying with glutamic acid caused a significant increase in the growth parameters , the effect of the interaction was significant also , the concentration 100 mg.L-1 glutamic acid and the the concentration 20 mM.L-1 hydrogen peroxide gave the best values for palnt hight , dry weight , the content of nitrogen , phosphorus , potassium , the protein concentration of vegetative part and plant seed . While the concertration 50 mg.L-1 glutamic acid and the concertration 20 mM.L-1 hydrogen peroxide gave the best values for chlorophyll a , b , total chlorophyll , the activity of the enzymes superoxide dismutase , peroxidase , the content of proline acid and the yield as No. of capsuls . plant-1 , Wt. of capsuls(gm) . plant-1 , Wt. of seeds(gm). Plant -1 , Biological yield (gm) . Plant -1 .