Antifungal Activity of Propolis Ethanol Extract Against Botrytis cinerea, Altrnaria sp Which Caused Many Plant Diseases

Abstract

  This study was conducted to determine the effect of different concentrations of   ethanol  extract of propolis against two  fungi Botrytis cinerea, Altrnaria sp.      The most important chemical constituents of propolis diagnosed by the Infra Red Spectroscopy, were the  Flavonoids  specificaly.       Results showed a high antifungal activity of ethanol  extract of propolis, evidently with a high concentration  of extract (5) mg\ml, which completely inhibited the radial growth on both  solid and liquid media (PDA &PD Broth ) of both fungi .         We observed that , ethanol  extract propolis at concentration of 5 mg\ml were sprayed on experimental infection on some  plants (eggplant leavers & cucumber ) which were effected by fungal. The spraying of   ethanol  extract of propolis before 24, 48 hour  prevented development  of disease and made a good protection of the mentioned plants from being infected with this fungus. While, the ability of  fungus to grow after 24, 48 hours from ethanol  extract of propolis treatment was reduce markedly as compared with control ,such treatment show  a slow growth of pathogen infect.