Eco-friendly Green Synthesis of Zinc Oxide Nanoparticles from different Plant Extracts: Structural and Optical Characterization

Raghad A. Rasheed; Maysoon F.A.  Alias

doi:10.30526/39.2.4312

Authors

Raghad A. Rasheed Department of Physics, College of Sciences, University of Baghdad, Baghdad, Iraq https://orcid.org/0009-0000-9735-8452
Maysoon F.A. Alias Department of Physics, College of Sciences, University of Baghdad, Baghdad, Iraq https://orcid.org/0000-0002-4118-6281

DOI:

https://doi.org/10.30526/39.2.4312

Keywords:

Green synthesis, Zinc oxide Nanoparticles, Structure, Morphology and optical properties

Abstract

To create nanoparticles of zinc oxide (ZnO), a green chemistry method was used. Using Cordia myxa (ZnO-C) and Ziziphus spina (ZnO-Z) extracts works like natural reduction and stabilization properties. The green process provided enhanced phytochemical interactions, which facilitated nanoparticle formation and improved stability. The concentrations of Zn and O elements were validated using energy-dispersive X-ray spectroscopy. The ZnO-C and ZnO-Z polycrystalline structures with a hexagonal wurtzite phase were validated by X-ray diffraction (XRD), where the average crystalline size is about 17.54 nm for ZnO-C, while for ZnO-Z it is equal to 16.809 nm. Field emission scanning electron microscopy (FESEM) micrograph of ZnO-C observed to be spherical, aggregated, and ranging between 35 and 52 nm in size, whereas the FESEM micrograph of ZnO-Z showed slightly larger particles ranging from 35 to 55 nm, which were also spherical to quasi-spherical particles with a tendency to form aggregated clusters. The optical properties of ZnO nanoparticles were examined using UV-Vis spectroscopy, indicating that ZnO-C has a 3.23 eV band gap, while for ZnO-Z it is 3.37 eV. As a result, ZnO nanoparticles have been confirmed to be created using an ecofriendly method for various applications

Author Biographies

Raghad A. Rasheed , Department of Physics, College of Sciences, University of Baghdad, Baghdad, Iraq

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Maysoon F.A. Alias, Department of Physics, College of Sciences, University of Baghdad, Baghdad, Iraq

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References

1. Mashrai A, Khanam H, Aljawfi RN. Biological synthesis of ZnO nanoparticles using C. albicans and studying their catalytic performance in the synthesis of steroidal pyrazolines. Arab J Chem. 2017; 10: 1530-1536.‏ https://doi.org/10.1016/j.arabjc.2013.05.004

2. Naser DK, Abbas AK, Aadim KA. Zeta potential of Ag, Cu, ZnO, CdO and Sn nanoparticles prepared by pulse laser ablation in liquid environment. Iraqi J Sci. 2020; 61(10): 2570-2581. https://doi.org/10.24996/ijs.2020.61.10.13

3. Rajabi HR, Naghiha R, Kheirizadeh M, Sadatfaraji H, Mirzaei A, Alvand ZM. Microwave assisted extraction as an efficient approach for biosynthesis of zinc oxide nanoparticles: synthesis, characterization, and biological properties. Mater Sci Eng C. 2017; 78:1109-1118.‏ https://doi.org/10.1016/j.msec.2017.03.090

4. Ramanujam K, Sundrarajan M. Antibacterial effects of biosynthesized MgO nanoparticles using ethanolic fruit extract of Emblica officinalis. J Photochem Photobiol B. 2014; 141: 296-300.‏ https://doi.org/10.1016/j.jphotobiol.2014.09.011

5. Rajeshkumar S, Naik P. Synthesis and biomedical applications of cerium oxide nanoparticles–a review. Biotechnol Rep. 2018; 17: 1-5.‏ https://doi.org/10.1016/j.btre.2017.11.008

6. Dobrucka R. Synthesis of titanium dioxide nanoparticles using Echinacea purpurea herba. Iran J Pharm Res. 2017; 16(2): 756-762.‏

7. Batool M, Khurshid S, Qureshi Z, Daoush WM. Adsorption, antimicrobial and wound healing activities of biosynthesised zinc oxide nanoparticles. Chem Pap. 2020; 75(3):893-907.‏ https://doi:10.1007/s11696-020-01343-7

8. Sarkar S, Ponce NT, Banerjee A, Bandopadhyay R, Rajendran S, Lichtfouse E. Green polymeric nanomaterials for the photocatalytic degradation of dyes: a review. Environ Chem Lett. 2020; 18(5): 1569-1580.‏ https://doi:10.1007/s10311-020-01021-w

9. Selmi M, Chaabouni F, Abaab M, Rezig B. Studies on the properties of sputter-deposited Al-doped ZnO films. Superlattices Microstruct. 2008; 44(3):268-275.‏ https://doi.org/10.1016/j.spmi.2008.06.005

10. Rasheed RA, Alias MFA. The role of aluminum doping on structural and optical properties of ZnO thin films prepared by PLD. IOP Conference Series: Mater Sci Eng. 2020; 757(1): 012056. https://doi.org/10.1088/1757-899X/757/1/012056

11. Zhaoyang W, Liyuan S, Lizhong H. Effect of laser repetition frequency on the structural and optical properties of ZnO thin films by PLD. Vacuum. 2010; 85(3): 397-399.‏ https://doi.org/10.1016/j.vacuum.2010.07.015

12. Silva RF, Zaniquelli ME. Aluminium-doped zinc oxide films prepared by an inorganic sol–gel route. Thin Solid Films. 2004; 449(1-2): 86-93. https://doi.org/10.1016/S0040-6090(03)01405-6

13. ‏Muslim AM, Naji IS. Antibacterial Activity of Prepared Zinc Oxide Nanoparticles by Eco-Friendly Method Using Olive Leaves and Roselle Flower Extracts. Int J Nanosci. 2024; 23(6): 2450011. https://doi.org/10.1142/S0219581X2450011X

14. Jiang JPiJ, Cai J . The advancing of zinc oxide nanoparticles for biomedical applications. Bioinorg Chem Appl. 2018; 1: 1062562. https://doi.org/10.1155/2018/1062562

15. Madhumitha G, Elango G, Roopan SM. Biotechnological aspects of ZnO nanoparticles: overview on synthesis and its applications. Appl Microbiol Biotechnol. 2016; 100(2): 571-581.‏ https://doi.org/10.1007/s00253-015-7108-x

16. Ali M, Ikram M, Ijaz M, Ul-Hamid A, Avais M, Anjum AA. Green synthesis and evaluation of n-type ZnO nanoparticles doped with plant extract for use as alternative antibacterial. Appl Nanosci. 2020; 10(10): 3787-3803.‏ https://doi.org/10.1007/s13204 020-01451-6

17. Busch G, Schade H, Birman JL. Lectures on solid state physics. Pergamon Press. 1st ed. 1976: 538. ‏

18. Umamaheswari A, Prabu SL, John SA. Puratchikody A. Green synthesis of zinc oxide nanoparticles using leaf extracts of Raphanus sativus var. Longipinnatus and evaluation of their anticancer property in A549 cell lines. Biotechno Rep. 2021; 29: e00595.‏ https://doi.org/10.1016/j.btre. 2021.e00595

19. Elavarasan N, Kokila K, Inbasekar G, Sujatha V. Evaluation of photocatalytic activity, antibacterial and cytotoxic effects of green synthesized ZnO nanoparticles by Sechium edule leaf extract. Res Chem Intermed. 2017; 43(5): 3361-3376.‏ https://doi.org/10.1007/s11164-016-2830-2

20. Aminuzzaman M, Ying LP, Goh WS, Watanabe A. Green synthesis of zinc oxide nanoparticles using aqueous extract of Garcinia mangostana fruit pericarp and their photocatalytic activity. Bull Mater Sci. 2018; 41(2): 1-10.‏ https://doi.org/10.1007/s12034-018 1568-4

21. Greenland K. Measurement and control of the thickness of thin films. Vacuum. 1952; 2(3): 216-230. https://doi.org/10.1016/0042-207X(52)90359-X

22. Gupta V. Mechanical techniques for the measurements of film thickness. Ch 11; Thin Film Science and Technology. 2026.

23. Nagaraja K, Hwan OT. Green synthesis of multifunctional zinc oxide nanoparticles from cordia myxa gum; and their catalytic reduction of nitrophenol, anticancer and antimicrobial activity. Int J Bio. Macromol. 2023; 253: 126788.‏ https://doi.org/10.1016/j.ijbiomac.2023.126788

24. Alharthi MN, Ismail I, Bellucci S, Salam, M A. Green synthesis of zinc oxide nanoparticles by Ziziphus jujuba leaves extract: Environmental application, kinetic and thermodynamic studies. J Phys Chem of Solids. 2021; 158: 110237.‏ https://doi.org/10.1016/j.jpcs.2021.110237

25. Molla A, Hoque M M, Richi FT, Kabir MH, Alam S, Ahmed N U, Emon NU, Shao C, Zeng C, Wang S, Geng P, Mamun, A. A. Biosynthesis and characterization of ZnO nanoparticles using citrus reticulata peel followed by photocatalytic, antibacterial, and antioxidative nanotherapeutic attributes assessment supported by computer simulation. Int J Nanomedicine. 2025; 20: 4803.‏ https://doi.org/10.2147/IJN.S493905

26. Hameed H, Waheed A, Sharif MS, Saleem M, Afreen A, Tariq M, Kamal A, A.Al-onazi W, A.Al Farraj D, Ahmed S, Mahmoud RM . Green synthesis of zinc oxide (ZnO) nanoparticles from green algae and their assessment in various biological applications. Micromach. 2023; 14(5): 928.‏ https://doi.org/10.3390/mi14050928

27. Barzinjy AA, Azeez HH. Green synthesis and characterization of zinc oxide nanoparticles using Eucalyptus globulus Labill. leaf extract and zinc nitrate hexahydrate salt. SN Appl Sci. 2020; 2(5): 991.‏ https://doi.org/10.1007/s42452-020-2813-1

28. Sowa H, Ahsbahs H. High-pressure X-ray investigation of zincite ZnO single crystals using diamond anvils with an improved shape. J Appl Crystallogr. 2006; 39(2): 169-175.‏ https://doi.org/10.1107/S0021889805042457

29. Al-Saadi TM. Investigating the structural and magnetic properties of nickel oxide nanoparticles prepared by precipitation method. Ibn Al-Haitham J Pure Appl Sci. 2022; 35(4): 94-103. https://doi.org/10.30526/35.4.2872

30. Janaki AC, Sailatha E, Gunasekaran S. Synthesis, characteristics and antimicrobial activity of ZnO nanoparticles. Spectrochim Acta A Mol Biomol Spectrosc. 2015; 144: 17-22.‏ https://doi.org/10.1016/j.saa.2015.02.041

31. Abdelbaky AS, Abd El-Mageed TA, Babalghith AO, Selim S, Mohamed AM. Green synthesis and characterization of ZnO nanoparticles using Pelargonium odoratissimum (L.) aqueous leaf extract and their antioxidant, antibacterial and anti-inflammatory activities. Antioxid. 2022; 11(8): 1444.‏ https://doi.org/10.3390/antiox11081444

32. Nhu VTT, Dat ND, Tam LM, Phuong NH. Green synthesis of zinc oxide nanoparticles toward highly efficient photocatalysis and antibacterial application. Beilstein J Nanotechnol. 2022; 13(1): 1108-1119.‏ https://doi.org/10.3762/bjnano.13.94

33. Alharthi MN, Ismail I, Bellucci S, Jaremko M, Abo-Aba SE, Abdel Salam M. Biosynthesized zinc oxide nanoparticles using Ziziphus jujube plant extract assisted by ultrasonic irradiation and their biological applications. Separations. 2023; 10(2): 78.‏ https://doi.org/10.3390/separations10020078

34. Karam ST, Abdulrahman AF. Green synthesis and characterization of ZnO nanoparticles by using thyme plant leaf extract. Photonics. 2022; 9(8): 594. https://doi.org/10.3390/photonics9080594

35. Fouladi-Fard R, Aali R, Mohammadi-Aghdam S, Mortazavi-derazkola S. The surface modification of spherical ZnO with Ag nanoparticles: A novel agent, biogenic synthesis, catalytic and antibacterial activities. J Chem. 2022; 15(3): 103658.‏ https://doi.org/10.1016/j.arabjc.2021.103658

36. MuthuKathija M, Badhush MSM, Rama V. Green synthesis of zinc oxide nanoparticles using Pisonia Alba leaf extract and its antibacterial activity. Appl Surf Sci. Adv.2023; 15:100400.‏ https://doi.org/10.1016/j.apsadv.2023.100400

37. Shoman NA, Salama A, Abbas FG, Mourad HH, Abbas HA. LC-MS/MS profiling and immunomodulatory potential of green-synthesized zinc oxide nanoparticles using Cordia sebestena leaves against chromium-induced acute lung injury in rats. J Drug Deliv Sci Technol. 2025; 106: 106750.‏ https://doi.org/10.1016/j.jddst.2025.106750‏

38. Saif S, Tahir A, Asim T, Chen Y, Khan M, Adil SF. Green synthesis of ZnO hierarchical microstructures by Cordia myxa and their antibacterial activity. Saudi J Biol Sci. 2019; 26(7): 1364-1371.‏ https://doi.org/10.1016/j.sjbs.2019.01.004