Flow Injection Spectrophotometric Indirect Assay of Amlodipine Besylate Using Crystal Violet Agent

Kawan  M.  Awad; Bushra B.  Qassim

doi:10.30526/39.2.4320

Authors

Kawan M. Awad Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq https://orcid.org/0009-0006-7770-5430
Bushra B. Qassim Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq https://orcid.org/0000-0003-3876-3161

DOI:

https://doi.org/10.30526/39.2.4320

Keywords:

Amlodipine Besylate, Crystal violet, Flow injection, Merging zone

Abstract

Amlodipine Besylate (AMB) was determined in pharmaceutical and biological samples using a continuous-flow injection technique characterized by simplicity, rapidity, cost-effectiveness, sensitivity, and a broad linear range. This study aims to develop and evaluate a flow injection analysis method for the rapid and reliable determination of Amlodipine in chemical and biological samples using minute quantities. The suggested method is based on the oxidation of the drug with a potassium bromide-potassium bromate mixture, followed by reaction with crystal violet as a reagent to produce a blue product, the absorbance of which was measured at 592 nm. The coefficient of determination (R2) is 0.9978, and the calibration curve's linear range is 10-250 μg/mL. The limit of detection for the standard was 2.46 μg/mL, the recovery was 99.81%, and the relative standard deviation was 2.4%. The proposed method was successfully used to estimate AMB in biological and pharmaceutical samples. Compared with those obtained using a United States Pharmacopeia reference method, the results were favorable, and at the 95% confidence level, there was no discernible difference in accuracy and precision

Author Biographies

Kawan M. Awad, Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq

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Bushra B. Qassim, Department of Chemistry, College of Science, University of Baghdad, Baghdad, Iraq

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