Detection of tox A gene in Pseudomonas aeruginosa that isolates from different clinical cases by using PCR.

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Rana M. Abdullah Al-Shwaikh
Abbas Falih Alornaaouti

Abstract

       Current study obtained (75) isolate of Pseudomonas aeruginosa collected from different cases included : 28 isolates from otitis media, 23 isolates from burn infections, 10 isolates from wound infections, 8 isolates from urinary tract infections and 6 isolates from blood, during the period between 1/9/2014 to 1/11/2014


       The result revealed that the tox A gene was present in 54 isolates (72%) of Pseudomonas aeruginosa. The gel electrophoresis showed that the molecular weight of tox A gene was 352 bp. The result shows 17 isolates (60.71%) from otitis media has tox A gene, 18 isolates (78.26%) from burn followed by 8 isolate (80%) from wound infection and 5 isolates (62.5%) from urinary tract infection , finally 6 isolates (100%) from blood have this gene.

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How to Cite
Al-Shwaikh, R. M. A., & Alornaaouti, A. F. (2018). Detection of tox A gene in Pseudomonas aeruginosa that isolates from different clinical cases by using PCR. Ibn AL-Haitham Journal For Pure and Applied Sciences, 2017(IHSCICONF), 26–30. https://doi.org/10.30526/2017.IHSCICONF.1767
Section
biology